Structural, textural and protein adsorption properties of kaolinite and surface modified kaolinite adsorbents
Structural, textural and protein adsorption properties of kaolinite and surface modified kaolinite adsorbents
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DOI：
10.1016/j.clay.2013.12.027
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发表时间：
2014-03-01
影响因子：
5.6
通讯作者：
Diaz, M.
中科院分区：
文献类型：
--
作者：
Duarte-Silva, R.;Villa-Garcia, M. A.;Diaz, M.
研究了粘土沉积沉积物中的高岭石、高岭石热脱羟基法得到的偏高岭石以及高岭石与硅烷偶联剂叔丁基二甲基氯硅烷反应制得的有机衍生物的结构、结构和蛋白质吸附性能。在室温和pH 5.0条件下，通过吸附实验研究了α -乳清蛋白（A-LA）、牛血清白蛋白（BSA）和β -乳球蛋白（B-LG）蛋白的保留能力以及蛋白结合的相互作用性质。蛋白质的吸附能力和选择性明显依赖于吸附剂表面的化学性质和结构性质。高岭石是a - la和BSA的强吸附剂，对B-LG具有很高的亲和力。偏高岭石对A-LA和B-LG的截留能力较好，但对BSA的截留能力不强。有机-高岭石杂化物对BSA和A-LA的吸附能力显著提高。FTIR结果表明，吸附剂表面与多肽之间不存在氢键。蛋白质结合的相互作用与吸附剂表面的亲疏水性和蛋白质的氨基酸组成密切相关，吸附模式还应考虑位阻效应。(C) 2014 Elsevier B.V.版权所有
The structural, textural and protein adsorption properties of kaolinite from clay sedimentary deposits, metakaolinite obtained by thermal dehydroxylation of kaolinite, and the organic derivative prepared by reacting kaolinite with the silane coupling agent tert-butyldimethylchlorosilane, were studied. The retention capacities for the proteins alpha-lactalbumin (A-LA), bovine serum albumin (BSA) and beta-lactoglobulin (B-LG) and the nature of the interactions responsible for protein binding were studied by adsorption experiments, performed at room temperature and pH 5.0. The protein adsorption capacity and the selectivity show a clear dependence on the chemical nature of the adsorbents surface and on the textural properties. Kaolinite behaves as a strong adsorbent for A-LA and BSA, and exhibits a very high affinity for B-LG. Metakaolinite shows good retention capacity for A-LA and B-LG, but does not retain significant amounts of BSA. The adsorption capacity of the organo-kaolinite hybrid considerably increases for BSA and A-LA. FTIR results indicate the absence of hydrogen bonding between the adsorbents surface and the polypeptides. The interactions responsible for protein binding are closely related to the hydrophilic or hydrophobic character of the adsorbent surface and the amino acid composition of the proteins, steric effects also should be considered for the adsorption patterns. (C) 2014 Elsevier B.V. All rights reserved.