Development of the automated gold-linked electrochemical immunoassay system for blood monitoring,
Development of the automated gold-linked electrochemical immunoassay system for blood monitoring,
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开发用于血液监测的自动化金联电化学免疫分析系统，
DOI：
10.1007/s00542-013-1772-5
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yuzuru Takamura,
中科院分区：
文献类型：
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作者：
Suguru Shiohara;Miyuki Chikae;Yoshiaki Ukita;Hiromi Ushijima;Yuzuru Takamura,
本文报道了全自动微型化免疫分析系统的研制。该系统由邮票大小的微芯片和紧凑的（明信片大小的足迹）微芯片驱动器。为了实现简单的样品加载到微芯片，表面改性的聚二甲基硅氧烷（PDMS）的开发，和改性表面的寿命长达9天的确认。该微芯片只需消耗一滴血（2 μl），通过毛细管作用实现样品的加载和计量，与采血针采血法兼容。使用全血样品在15分钟内证明了系统中的全自动免疫测定方案。最后，在开发的系统中成功地证明了抗原（胰岛素）的全自动检测。
This paper reports the development of fully automated miniaturized immunoassay system. The system consist of postage stamp sized microchip and compact (post card sized foot print) microchip driver. To realize easy sample loading into the microchip, surface modification of polydimethylsiloxane (PDMS) was developed, and life time of the modified surface up to 9 days is confirmed. The microchip just consumes a droplet of blood (2 μl) and the loading and metering of the sample is realized by capillary action, therefore the microchip is compatible with blood collection method by using lancet needle. Fully automated immunoassay protocol in the system is demonstrated within 15 min using whole blood sample. Finally, fully automated detection of antigen (insulin) was successfully demonstrated in the developed system.