Correlation of the Mitochondrial Activity of Two‐Cell Embryos Produced In Vitro and the Two‐Cell Block In Kunming and B6C3F1 Mice
Correlation of the Mitochondrial Activity of Two‐Cell Embryos Produced In Vitro and the Two‐Cell Block In Kunming and B6C3F1 Mice
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DOI：
10.1002/ar.20890
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发表时间：
2009-05
期刊：
影响因子：
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通讯作者：
Shie Wang;Cuiying Lin;H. Shi;Meirong Xie;Weiyu Zhang;J. Lv
中科院分区：
文献类型：
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作者：
Shie Wang;Cuiying Lin;H. Shi;Meirong Xie;Weiyu Zhang;J. Lv
通过比较昆明小鼠（KM）和B6 C3 F1小鼠体外生产的两细胞胚胎，研究了早期胚胎阻滞与发育和线粒体活性之间的相关性。从两种杂交种（与KM雄性交配的雌性KM小鼠和与KM雄性交配的雌性B6 C3 F1小鼠）中获得单细胞胚胎，并在M16培养基中培养84小时。在KM和B6 C3 F1双细胞胚胎中测量线粒体膜电位、ATP含量和活性氧水平。线粒体膜电位和ATP含量也测定在KM和B6 C3 F1中期II卵。结果表明，在培养的KM胚胎中观察到两细胞阻滞，而在B6 C3 F1胚胎中未观察到。KM两细胞胚线粒体膜电位和ATP含量显著低于B6 C3 F1两细胞胚（P < 0.01）。有趣的是，KM两细胞胚胎的活性氧水平显著低于B6 C3 F1胚胎（P < 0.01）。KM和B6 C3 F1中期II卵的线粒体膜电位和ATP含量无差异。由此得出结论，KM小鼠具有早期两细胞胚胎阻滞，并且可能的“阻滞”机制是这些胚胎中较低的线粒体膜电位和ATP含量。这些结果为克服早期胚胎发育障碍提供了一种新的方法，即操纵线粒体活性。Anat Rec，292：661-669，2009.© 2009 Wiley‐利斯公司
The correlation between the early embryonic block to development and mitochondrial activity was investigated comparing two‐cell embryos produced in vitro from Kunming (KM) and B6C3F1 mice. One‐cell embryos were obtained from two species of hybrids (female KM mice mated with KM males and female B6C3F1 mice mated with KM males) and cultured for 84 hr in M16 media. The mitochondrial membrane potential, ATP content, and reactive oxygen species levels were measured in the resulting KM and B6C3F1 two‐cell embryos. Mitochondrial membrane potential and ATP content were also determined in KM and B6C3F1 metaphase II eggs. The results showed that the two‐cell block was observed in cultured KM embryos but not in B6C3F1 embryos. Mitochondrial membrane potential and ATP content of KM two‐cell embryos were significantly lower than in B6C3F1 two‐cell embryos (P < 0.01). Interestingly, the reactive oxygen species levels of KM two‐cell embryos were significantly lower than their B6C3F1 counterparts (P < 0.01). There was no difference in mitochondrial membrane potential and ATP content between KM and B6C3F1 metaphase II eggs. It is concluded that KM mice have an early two‐cell embryo block and that a possible “blocking” mechanism is the lower mitochondrial membrane potential and ATP content in these embryos. The results suggest a new approach for overcoming early embryonic development block, that of manipulating mitochondrial activity. Anat Rec, 292:661–669, 2009. © 2009 Wiley‐Liss, Inc.