Microtubule depolymerization at high pressure
Microtubule depolymerization at high pressure
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DOI：
10.1111/j.1749-6632.2009.05411.x
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Terazima, Masahide
中科院分区：
文献类型：
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作者：
Nishiyama, Masayoshi;Shimoda, Yoshiki;Terazima, Masahide
我们进行了体外试验，以可视化压力对微管丝状结构的影响。紫杉醇稳定的微管被拴在高压室的观察窗上的驱动蛋白马达上。当对样品溶液施加压力时，所有微管从两端开始缩短。长度的变化是恒定的，随着时间的推移，无论微管的极性。微管的缩短速率随压力呈指数增加，活化体积为~ 100 mL/mol，与体内研究一致。这些结果表明，压力的应用直接削弱微管蛋白分子之间的分子间相互作用。
We performed in vitro assays to visualize the effects of pressure on the filamentous structure of microtubules. Taxol-stabilized microtubules were tethered to kinesin motors on the observation window of a high-pressure chamber. When pressure was applied to the sample solution, all of the microtubules started to shorten from both ends. The length changes were constant over time, irrespective of the microtubule polarity. The shortening rate of microtubules increased exponentially with pressure, and the activation volume was -100 mL/mol, consistent with in vivo studies. These results show that application of pressure works directly to weaken the intermolecular interactions between tubulin molecules.