The human DINB1 gene encodes the DNA polymerase Polθ
The human DINB1 gene encodes the DNA polymerase Polθ
复制标题
DOI：
10.1073/pnas.97.8.3838
复制
发表时间：
2000-04-11
影响因子：
11.1
通讯作者：
Prakash, L
中科院分区：
文献类型：
--
作者：
Johnson, RE;Prakash, S;Prakash, L
人DINB 1基因与大肠杆菌dinB基因具有高度同源性。在这里，我们纯化hDINB 1编码的蛋白质，并表明它是一个DMA聚合酶。因为hDinB 1是第八个被描述的真核DNA聚合酶，我们将其命名为DNA聚合酶（Pol）θ。hPol θ不能绕过顺式-顺式胸腺嘧啶-胸腺嘧啶二聚体，也不能绕过（6-4）光产物或脱碱基位点。我们还通过稳态动力学分析检测了hPol theta对未受损DNA模板的保真度，发现hPol theta错误掺入脱氧核苷酸的频率约为10（-3）至10（-4）。我们讨论了hPol theta的保真度与其无法绕过DNA损伤之间的关系。
The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DMA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) theta. hPol theta is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6-4) photoproduct or an abasic site. We also examine the fidelity of hPol theta on nondamaged DNA templates by steady-state kinetic analyses and find that hPol theta misincorporates deoxynucleotides with a frequency of about 10(-3) to 10(-4). We discuss the relationship between the fidelity of hPol theta and its inability to bypass DNA damage.