The Eupentacta fraudatrix transcriptome provides insights into regulation of cell transdifferentiation
The Eupentacta fraudatrix transcriptome provides insights into regulation of cell transdifferentiation
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DOI：
10.1038/s41598-020-58470-0
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发表时间：
2020-01-30
影响因子：
4.6
通讯作者：
Dolmatov, Igor Yu
中科院分区：
文献类型：
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作者：
Boyko, Alexey, V;Girich, Alexander S.;Dolmatov, Igor Yu
海参Eupentacta fraudatrix是研究再生机制的独特生物。此外，E. fraudatrix可以迅速恢复其身体的部分和整个器官系统，但目前还没有关于干细胞参与这一过程的数据。相反，已经反复证实，这一过程仅仅是由于终末分化细胞的转化。在这项研究中，我们研究了基因表达的变化，在肠道再生的海参E。骗子用Illumina测序仪（San Diego，CA，USA）对三个再生阶段的肠原基以及正常肠的转录组进行测序。我们鉴定了14，617个海胆蛋白同源物，其中308个是转录因子。在分析了再生过程中基因表达的动态和它们参与的生物过程的图谱后，我们确定了11个因子：Ef-EGR1、Ef-ELF、Ef-GATA 3、Ef-ID 2、Ef-KLF 1/2/4、Ef-MSC、Ef-PCGF 2、Ef-PRDM 9、Ef-SNAI2、Ef-TBX20和Ef-TCF 24。除TCF 24外，它们都参与其他动物的再生、发育、上皮-间质转化和免疫应答。我们认为这些转录因子也可能参与海参体腔上皮细胞向肠上皮细胞的转分化。
The holothurian Eupentacta fraudatrix is a unique organism for studying regeneration mechanisms. Moreover, E. fraudatrix can quickly restore parts of its body and entire organ systems, yet at the moment, there is no data on the participation of stem cells in the process. To the contrary, it has been repeatedly confirmed that this process is only due to the transformation of terminally differentiated cells. In this study, we examine changes in gene expression during gut regeneration of the holothurian E. fraudatrix. Transcriptomes of intestinal anlage of the three stages of regeneration, as well as the normal gut, were sequenced with an Illumina sequencer (San Diego, CA, USA). We identified 14,617 sea urchin protein homologs, of which 308 were transcription factors. After analysing the dynamics of gene expression during regeneration and the map of biological processes in which they participate, we identified 11 factors: Ef-EGR1, Ef-ELF, Ef-GATA3, Ef-ID2, Ef-KLF1/2/4, Ef-MSC, Ef-PCGF2, Ef-PRDM9, Ef-SNAI2, Ef-TBX20, and Ef-TCF24. With the exception of TCF24, they are all involved in the regeneration, development, epithelial-mesenchymal transition, and immune response in other animals. We suggest that these transcription factors may also be involved in the transdifferentiation of coelomic epithelial cells into enterocytes in holothurians.