Isoform-specific expression of BAG-1 in mouse development
Isoform-specific expression of BAG-1 in mouse development
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DOI：
10.1016/s0925-4773(99)00284-1
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发表时间：
2000-03-01
影响因子：
2.6
通讯作者：
Cato, ACB
中科院分区：
文献类型：
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作者：
Crocoll, A;Blum, M;Cato, ACB
BAG-1 是一个蛋白质家族，在培养细胞中具有多种活性，从防止程序性细胞死亡到调节类固醇激素作用。 Bag-1 mRNA 通过使用替代翻译起始位点编码至少三种蛋白质（BAG-1L、BAG-1M 和 BAG-1）。为了评估这些因子的体内功能，我们使用原位杂交和免疫组织化学技术来确定 Bag-1 转录物和蛋白质在小鼠发育过程中的分布。 Bag-1 mRNA 在多个器官中被发现，其中软骨组织表现出最高的表达水平。其中一些位点的表达水平在发育过程中下调。在免疫组织化学研究中，尽管观察到同工型特异性差异，但针对 BAG-1 蛋白的抗体对原位杂交研究中鉴定的所有位点进行了染色。 BAG-1L 特异性抗体早在性交后第 10.5 天就显示出普遍存在的染色，但在胚胎发生的后续阶段存在逐渐限制。相反，优先识别其他亚型的抗体仅对早期发育阶段的小鼠心肌进行染色，然后最终识别发育后期的其他器官。这些结果证明了 BAG-1 同工型在小鼠发育过程中的阶段和位点特异性表达。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
BAG-1 is a family of proteins with diverse activities that range in cultured cells from protection against programmed cell death through to regulation of steroid hormone action. At least three proteins (BAG-1L, BAG-1M and BAG-1) are encoded by the Bag-1 mRNA through the use of alternative translation-initiation sites. To assess the in vivo function of these factors, we have used in situ hybridization and immunohistochemical techniques to determine the distribution of Bag-1 transcript and proteins during mouse development. Bag-1 mRNA was identified in several organs with cartilaginous tissues showing the highest expression levels. The level of expression at some of these sites was downregulated during the course of development. In the immunohistochemical studies, antibodies directed against the BAG-1 proteins stained all the sites identified in the in situ hybridization studies although isoform-specific differences were observed. BAG-1L specific antibody showed ubiquitous staining as early as day 10.5 post-coitum but there was a progressive restriction during subsequent stages of embryogenesis. On the contrary, an antibody that preferentially recognized the other isoforms only stained the mouse myocardium in the early developmental stages before finally recognizing additional organs later on in development. These results demonstrate a stage- and site-specific expression of the BAG-1 isoforms during mouse development. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.