SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism
SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism
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SCOTflors：用于细胞代谢体内成像的小型、可共轭、正交和可调谐荧光团
DOI：
10.1002/ange.201900465
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发表时间：
2019
影响因子：
--
通讯作者：
Benson S
中科院分区：
文献类型：
--
作者：
Benson S
代谢物的运输和贩运对于活细胞的正确功能至关重要。然而，原位代谢成像研究受到缺乏荧光化学结构的阻碍，荧光化学结构允许在生理条件下以高的空间和时间分辨率直接监测小代谢物。 在本文中，我们将SCOTfluors描述为一种新型的小尺寸多色荧光团，用于活细胞和体内必需代谢物的真实的时间跟踪，并用于从不同来源的人类癌细胞中获取代谢谱。
The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein, we describe SCOTfluors as novel small‐sized multi‐colored fluorophores for real‐time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.