STIMULATION OF AROMATASE ACTIVITY BY FOLLICLE-STIMULATING-HORMONE IN RAT GRANULOSA-CELLS INVIVO AND INVITRO
STIMULATION OF AROMATASE ACTIVITY BY FOLLICLE-STIMULATING-HORMONE IN RAT GRANULOSA-CELLS INVIVO AND INVITRO
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DOI：
10.1210/endo-102-4-1275
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发表时间：
1978-01-01
期刊：
影响因子：
4.8
通讯作者：
HSUEH, AJW
中科院分区：
文献类型：
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作者：
ERICKSON, GF;HSUEH, AJW
以未成熟、去垂体、雌激素刺激的幼鼠为模型，研究卵泡刺激素(FSH)在颗粒细胞芳香酶活性中的作用。每天两次注射绵羊FSH后，颗粒细胞中芳香酶活性在24小时滞后期后被诱导。持续的FSH处理导致芳香酶活性进一步提高。48h后，该酶的活性与成人排卵前卵泡颗粒细胞中的酶活性相当。通过在含有这些激素的化学介质中体外培养颗粒细胞2天，研究了酶底物(雄烯二酮)以及纯化的FSH和LH对芳香酶活性的影响。在没有芳香酶底物的情况下，对照和促性腺激素处理的培养物对雌激素的分泌没有明显的刺激作用。然而，在酶底物(10-7M雄烯二酮)的存在下，高纯度的FSH(200 ng/ml)，而不是促黄体生成素(200 ng/ml)，分别在第1天和第2天刺激雌激素的产生8.8倍和40倍。体外研究了FSH与芳香酶活性的剂量-反应关系。在雄烯二酮(10-7M)存在下，随着FSH浓度的增加(0.3-100 ng/ml)，1 ng/mlFSH和100 ng/mlFSH的雌激素产生呈剂量依赖性增加，分别增加6倍和80倍。FSH浓度为5 ng/ml才能达到雌激素半高水平，在未添加雄烯二酮的情况下，在培养2天内未观察到雌激素分泌的刺激作用，但当FSH处理后的培养细胞再与10-7M雄烯二酮一起孵育3h时，颗粒细胞雌激素的产生随FSH浓度的增加而增加，说明FSH单独激活了芳香化酶。FSH在体内和体外均能刺激大鼠颗粒细胞芳香化酶活性，且该过程具有时间和剂量依赖性。
The role of FSH [follicle stimulating hormone] in the activation of aromatase enzymes in granulosa cells was studied by using immature, hypophysectomized, estrogen-primed rats as a model system. After twice daily injections of ovine FSH, aromatase activity was induced in granulosa cells after a 24 h lag period. Continued FSH treatment caused a further increase in aromatase activity. After 48 h, the enzyme activity was comparable to that found in granulosa cells of adult preovulatory follicles. The effects of enzyme substrate (androstenedione) as well as purified FSH and LH upon aromatase activity were investigated in vitro by culturing granulosa cells for 2 days in a chemically defined medium containing these hormones. In the absence of aromatase substrate, no significant stimulation of estrogen secretion was observed in control and gonadotropin-treated cultures. However, in the presence of enzyme substrate (10-7 M androstenedione), highly purified FSH (200 ng/ml), but not LH (200 ng/ml), stimulated estrogen production 8.8-and 40-fold after 1 and 2 days, respectively. The dose-response relationship between FSH and aromatase activity was studied in vitro. In the presence of androstenedione (10-7 M), increasing concentrations of FSH (0.3-100 ng/ml) resulted in a dose-related increase in estrogen production with 1 ng and 100 ng/ml FSH, causing a 6- and 80-fold increase in estrogen production, respectively. The concentration of FSH required for half-maximum estrogen production was 5 ng/ml. Without added androstenedione, no stimulation of estrogen secretion was observed during the 2-day culture period; however, when these FSH-treated cultures were washed and then reincubated for 3 h with 10-7 M androstenedione, and FSH dose-related increase in estrogen production by the granulosa cells was observed, demonstrating that FSH alone activated the aromatase enzymes. FSH stimulates aromatase enzyme activity in rat granulosa cells in vivo and in vitro, and this process is time and dose dependent.