Nucleic acid delivery with microbubbles and ultrasound.
Nucleic acid delivery with microbubbles and ultrasound.
复制标题
DOI：
10.1016/j.addr.2014.01.009
复制
发表时间：
2014-06
影响因子：
16.1
通讯作者：
Klibanov, Alexander L.
中科院分区：
文献类型：
--
作者：
Rychak, Joshua J.;Klibanov, Alexander L.
基于核酸的治疗是药物传递研究的一个新兴领域。虽然早在几十年前就提出了超声增强转染的建议，但要达到合理的核酸递送效果，需要超声与核酸载体系统（微泡、脂质体、多聚体、病毒载体）相结合。微泡作为超声能量在靶细胞附近局部沉积的焦点，大大增强了超声穿孔。该方法的主要优点是在非超声非靶组织中转染量最小。微泡可以简单地与核酸载体共同施用，也可以修饰为携带核酸本身。嵌入气体或气体前体颗粒的脂质体也可用于携带核酸，通过超声波触发释放和传递核酸。在多种动物模型（心肌、实体瘤、骨骼肌、胰腺）中的成功测试证明了该技术在核酸药物输送方面的潜在用途。
Nucleic acid-based therapy is a growing field of drug delivery research. Although ultrasound has been suggested to enhance transfection decades ago, it took a combination of ultrasound with nucleic acid carrier systems (microbubbles, liposomes, polyplexes, viral carriers) to achieve reasonable nucleic acid delivery efficacy. Microbubbles serve as foci for local deposition of ultrasound energy near the target cell, and greatly enhance sonoporation. Major advantage of this approach is in the minimal transfection in the non-insonated non-target tissues. Microbubbles can be simply co-administered with the nucleic acid carrier or can be modified to carry nucleic acid themselves. Liposomes with embedded gas or gas precursor particles can also be used to carry nucleic acid, release and deliver it by the ultrasound trigger. Successful testing in a wide variety of animal models (myocardium, solid tumors, skeletal muscle, pancreas) proves the potential usefulness of this technique for nucleic acid drug delivery.
登录
查看更多内容
影响因子：
2.9
作者：
Carson, Andrew R.;McTiernan, Charles F.;Lavery, Linda;Hodnick, Abigail;Grata, Michelle;Leng, Xiaoping;Wang, Jianjun;Chen, Xucai;Modzelewski, Ruth A.;Villanueva, Flordeliza S.
通讯作者：
Villanueva, Flordeliza S.
影响因子：
5.1
作者：
通讯作者：
--
DOI：
10.1186/1756-9966-29-152
发表时间：
2010-11-23
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Chen ZY;Liang K;Qiu RX
通讯作者：
Qiu RX
影响因子：
5.1
作者：
Chen, S.;Ding, J.;Grayburn, P. A.
通讯作者：
Grayburn, P. A.
影响因子：
10.8
作者：
Delalande, Anthony;Bouakaz, Ayache;Pichon, Chantal
通讯作者：
Pichon, Chantal