Selective and sensitive detection of MiRNA-21 based on gold-nanorod functionalized polydiacetylene microtube waveguide.
Selective and sensitive detection of MiRNA-21 based on gold-nanorod functionalized polydiacetylene microtube waveguide.
复制标题
基于金纳米棒功能化聚二乙炔微管波导的miRNA-21选择性灵敏检测
DOI：
10.1016/j.bios.2016.05.019
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发表时间：
2016-11-15
影响因子：
12.6
通讯作者：
Lakowicz JR
中科院分区：
文献类型：
--
作者：
Zhu Y;Qiu D;Yang G;Wang M;Zhang Q;Wang P;Ming H;Zhang D;Yu Y;Zou G;Badugu R;Lakowicz JR
在复杂的生物环境中快速、高选择性和灵敏地检测miRNA已经引起了人们的广泛关注。在这里，我们描述了一种新的两步法构建金纳米棒功能化聚二乙炔(PDA)微管用于miRNA检测。在PDA微管中，由于具有一维(1D)波导的性质，激发位置和发射出耦合位置相距较远，从而有助于降低生物样品的自发荧光贡献。利用特殊设计的脚趾介导的链置换反应，可以可靠和选择性地区分序列同源性高的miRNA序列。基于浓缩浓缩效应，所提出的PDA微管系统的检测下限低至0.01nM，可直接用于检测人血清中的疾病特异性miRNA靶标。这种PDA微管波导系统可以进一步集成到芯片中，用于微创、便携式临床诊断设备的潜在应用。
Development of rapid, highly selective and sensitive miRNA detection in a complex biological environment has attracted considerable attention. Herein, we describe a novel two step method to construct gold-nanorod functionalized polydiacetylene (PDA) microtube for miRNA detection. In PDA microtube, with a one-dimensional (1D) waveguide nature, the excitation position and emission out-coupling position are far apart, thus helpful in reducing contribution of auto-fluorescence from biological sample. The use of specially designed toehold-mediated strand displacement reaction enables the reliable and selective discrimination of miRNA sequences with high sequence homology. Based on the condensing enrichment effect, the detection limit of the proposed PDA microtube system is as low as 0.01 nM, and it can be applied directly to detect disease-specific miRNA targets in human serum. This PDA microtube waveguide system can be further integrated into the chip for the potential applications in minimally invasive, portable clinical diagnostic equipment.