Bacteroides acidifaciens sp, nov., isolated from the caecum of mice

Bacteroides acidifaciens sp, nov., isolated from the caecum of mice
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DOI:
10.1099/00207713-50-1-145
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发表时间:
2000-01-01
影响因子:
2.8
通讯作者:
Itoh, K
Itoh, K
中科院分区:
生物学3区
文献类型:
--
作者:
Miyamoto, Y;Itoh, K

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在研究小鼠肠道细菌植物群的过程中,我们分离出了能降低含有菲尔德斯消化物的蛋白胨-酵母肉汤pH值的特征菌株。根据16 S rRNA序列,这些分离物被认为属于噬细胞-黄杆菌-拟杆菌门拟杆菌亚群中的拟杆菌簇。并被分成两组。它们的表型特征,即在20%胆汁中的生长、七叶皂苷水解、葡萄糖-6-磷酸脱氢酶(G6 PDH)和6-磷酸葡萄糖酸脱氢酶(6PGDH)活性,与“脆弱拟杆菌群”的表型特征相同。低水平的DNA-DNA杂交与模式菌株的拟杆菌集群证实了这些菌株的新物种的地位。拟将这些菌株命名为产酸拟杆菌(Bacteroides acidifaciens),其模式菌株为A40(T)(= JCM 105563(T))。
During studies of the bacterial flora in the intestines of mice, we isolated characteristic strains which lowered the ph of peptone-yeast broth containing Fildes' digest. Based on 16S rRNA sequence camparison, these isolates were considered to belong to the Bacteroides cluster in the bacteroides subgroup of the Cytophaga-Flavobacterium-Bacteroides phylum. and were divided into two groups. Their phenotypic characteristics, i.e. growth in 20% bile, aesculin hydrolysis, and glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activity, were the same as those of the 'Bacteroides fragilis group'. The low level of DNA-DNA hybridization with type strains in the Bacteroides cluster confirmed the novel species status of these isolates. It is proposed that these isolates be named Bacteroides acidifaciens, the type strain of which is A40(T)(= JCM 105563(T)).