The sucrose regulated transcription factor bZIP11 affects amino acid metabolism by regulating the expression of ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2
The sucrose regulated transcription factor bZIP11 affects amino acid metabolism by regulating the expression of ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2
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DOI：
10.1111/j.1365-313x.2007.03385.x
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发表时间：
2008-03-01
期刊：
影响因子：
7.2
通讯作者：
Smeekens, Sjef
中科院分区：
文献类型：
--
作者：
Hanson, Johannes;Hanssen, Micha;Smeekens, Sjef
转录因子bZIP11的翻译受到蔗糖的抑制，该过程涉及由bZIP11的5‘前导和其他植物碱性区域亮氨酸拉链(BZip)基因编码的高度保守的多肽。在生理蔗糖浓度下工作的特定信号通路可能通过反馈机制控制新陈代谢。在这篇文章中，通过瞬时增加的核bZIP11水平和全基因组表达分析来确定bZIP11的靶过程。BZIP11影响数百个基因的表达，这些基因被认为在生化途径和信号转导中具有功能。大约80%的被测基因的表达水平不受bZIP11启动子介导的bZIP11过表达的影响。这表明，已鉴定的基因中有20%似乎是bZIP11的生理相关靶标。天冬酰胺SYNTHETASE 1和Pro DEHYDROGENASE 2是快速激活的bZIP11靶标之一，其诱导不依赖于蛋白质翻译。在拟南芥原生质体中的瞬时表达实验表明，bZIP11依赖的天冬酰胺SYNTHETASE1基因的激活依赖于启动子中存在的G-box元件。BZIP11表达增加会导致脯氨酸水平降低，苯丙氨酸水平升高。提出了糖信号通过bZIP11转录因子控制氨基酸水平的模型。
Translation of the transcription factor bZIP11 is repressed by sucrose in a process that involves a highly conserved peptide encoded by the 5' leaders of bZIP11 and other plant basic region leucine zipper (bZip) genes. It is likely that a specific signaling pathway operating at physiological sucrose concentrations controls metabolism via a feedback mechanism. In this paper bZIP11 target processes are identified using transiently increased nuclear bZIP11 levels and genome-wide expression analysis. bZIP11 affects the expression of hundreds of genes with proposed functions in biochemical pathways and signal transduction. The expression levels of approximately 80% of the genes tested are not affected by bZIP11 promoter-mediated overexpression of bZIP11. This suggests that < 20% of the identified genes appear to be physiologically relevant targets of bZIP11. ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2 are among the rapidly activated bZIP11 targets, whose induction is independent of protein translation. Transient expression experiments in Arabidopsis protoplasts show that the bZIP11-dependent activation of the ASPARAGINE SYNTHETASE1 gene is dependent on a G-box element present in the promoter. Increased bZIP11 expression leads to decreased proline and increased phenylalanine levels. A model is proposed in which sugar signals control amino acid levels via the bZIP11 transcription factor.