EVIDENCE FOR A FUNCTIONAL LINK BETWEEN PROFILIN AND CAP IN THE YEAST SACCHAROMYCES-CEREVISIAE
EVIDENCE FOR A FUNCTIONAL LINK BETWEEN PROFILIN AND CAP IN THE YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0092-8674(81)90013-1
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发表时间：
1991-08-09
期刊：
影响因子：
64.5
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
VOJTEK, A;HAARER, B;WIGLER, M
CAP是酿酒酵母腺酰环化酶复合体的一种成分。N-末端结构域是蜂窝RAS响应所必需的。C-末端结构域的缺失与形态和营养缺陷有关。在这里，我们报告帽细胞随机萌发，肌动蛋白分布有缺陷。与CAP C-末端结构域缺失相关的形态和营养缺陷可通过编码Profilin的基因PFY的过表达来抑制，Profilin是一种肌动蛋白和多聚磷肌醇结合蛋白。缺乏PFY的细胞的表型与缺乏CAP C末端结构域的细胞相似。对突变的酵母Profilin和棘阿米巴Profilins的研究表明，Profilin抑制帽细胞的能力取决于其结合肌动蛋白的能力以外的另一种性质。这一特性可能是其结合多磷脂酰肌醇的能力。我们认为，CAP和Profilin在生长信号和细胞骨架重塑之间提供了联系。
CAP is a component of the S. cerevisiae adenylyl cyclase complex. The N-terminal domain is required for cellular RAS responsiveness. Loss of the C-terminal domain is associated with morphological and nutritional defects. Here we report that cap- cells bud randomly and are defective in actin distribution. The morphological and nutritional defects associated with loss of the CAP C-terminal domain are suppressed by overexpression of PFY, the gene encoding profilin, an actin- and polyphosphoinositide-binding protein. The phenotype of cells lacking PFY resembles that of cells lacking the CAP C-terminal domain. Study of mutated yeast profilins and profilins from Acanthamoeba suggests that the ability of profilin to suppress cap- cells is dependent upon a property other than, or in addition to, its ability to bind actin. This property may be its ability to bind polyphosphoinositides. We propose that CAP and profilin provide a link between growth signals and remodeling of the cellular cytoskeleton.