The development of estrogen receptor systems in the rat brain and pituitary: Postnatal development
The development of estrogen receptor systems in the rat brain and pituitary: Postnatal development
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大鼠大脑和垂体中雌激素受体系统的发育：出生后发育
DOI：
10.1016/0006-8993(79)90094-5
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
B. McEwen
中科院分区：
文献类型：
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作者：
N. MacLusky;C. Chaptal;B. McEwen
本文用~ 3 H标记的雌激素对大鼠脑雌激素受体系统进行了体外细胞质结合实验和体内细胞核实验。细胞质和核的措施给出了一个类似的图片雌激素受体水平的变化，从第2-3天到第25天的出生后的生活。垂体受体水平在第10天达到峰值，然后略有下降。在第8天至第15天之间，在下丘脑中观察到类似的峰值，但不太明显。在杏仁核中，受体的浓度保持相对恒定，除了在第10天左右略有增加。在视前区，受体水平在整个研究期间增加。在皮质中，受体水平在第3天和第10天之间增加，然后急剧下降，并从第15天起保持较低水平。在中脑和脑干中，受体水平在整个时期都保持较低水平。通过交换试验研究了细胞核中雌二醇对雌激素受体的占用。在出生后第2-3天，雌性幼仔的皮质或边缘结构（下丘脑、杏仁核、视前区）中均未观察到占用;在出生后第10-11天和第25-26天，在边缘结构中观察到约5%容量的低水平占用，但在雌性幼仔的皮质中未观察到。的可能性进行了讨论，这种雌激素来自睾酮通过芳构化。内源性循环雌二醇的受体占领的明显情况下进行了讨论，在新生儿期甲胎蛋白的存在和逐渐消失。所提供的数据显示，注射雌激素在新生大鼠中的半衰期长于3周龄动物，并且与雌二醇-17 β相比，雌激素（如己烯雌酚和莫雌酚）与甲胎蛋白结合不强，在较低剂量下可进入脑雌激素受体。
The postnatal development of the estrogen receptor system of the rat brain is described by means of data from in vitro cytosol binding assays and from in vivo cell nuclear experiments using3H-labeled estrogens. Cytosol and nuclear measures give a similar picture of the changes in estrogen receptor levels from day 2–3 up to day 25 of postnatal life. Pituitary receptor levels reach a peak at day 10 and then decline slightly. A similar, though less pronounced, peak was seen in hypothalamus between days 8 and 15. In the amygdala, the concentration of receptors remains relatively constant apart from a slight increase around day 10. In the preoptic area, receptor levels increase throughout the entire period studied. In the cortex receptor levels increase between days 3 and 10 and then decline precipitously and remain low from day 15 onwards. In the midbrain and brain stem receptor levels remain low throughout the entire period.Occupation of estrogen receptors by estradiol in cell nuclei was investigated by means of an exchange assay. No occupation was seen in either cortex or limbic structures (hypothalamus, amygdala, preoptic area) of female pups on postnatal days 2–3; low level occupancy amounting to around 5% of capacity was seen in limbic structures but not in cortex of females on postnatal days 10–11 and 25–26. The possibility is discussed that this estrogen arises from testosterone via aromatization. The apparent absence of receptor occupation by endogenous circulating estradiol is discussed in relation to the presence and progressive disappearance of alpha-fetoprotein in the neonatal period. Data is presented showing that injected estrogens have a longer half-life in newborn rats than in 3-week-old animals and that estrogens such as diethylstilbestrol and moxestrol, which do not bind strongly to alpha-fetoprotein, gain access to brain estrogen receptors at lower doses compared to estradiol-17β.
大脑的发育和可塑性
DOI：
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发表时间：
2022
期刊：
影响因子：
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作者：
通讯作者：
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