Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector
Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector
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DOI：
10.1248/cpb.45.737
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发表时间：
1997-04-01
影响因子：
1.7
通讯作者：
Kamiya, A
中科院分区：
文献类型：
--
作者：
Cho, Y;Matsuoka, N;Kamiya, A
本文建立了高效液相色谱-二极管阵列检测器法测定急性中毒患者血清和尿液中11种有机磷农药（敌敌畏、杀扑磷、柳硫磷、马拉硫磷、杀螟松、倍硫磷、二嗪农、乙硫酮、O-乙基O-（4-硝基苯基）苯基硫代膦酸酯（EPN）和毒死蜱）及其代谢产物3-甲基-3-硝基苯酚的方法。将乙腈脱蛋白后的生物样品的等分试样进样至C-18柱中，使用乙腈-水作为移动的相。在230 nm波长处，血清和尿液中的检出限为0.05 ~ 6.8 μ g/ml。该方法已成功地应用于两例急性中毒的实际病例。
We have developed a simple and rapid method for measuring 11 organophosphorus pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4-nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-3-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into a C-18 column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 mu g/ml at a wavelength of 230 nn. This method was successfully applied to two actual cases of acute poisoning.