Intracellular and Extracellular Biosynthesis of Antibacterial Silver Nanoparticles by Using Pseudomonas aeruginosa
Intracellular and Extracellular Biosynthesis of Antibacterial Silver Nanoparticles by Using Pseudomonas aeruginosa
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利用铜绿假单胞菌在细胞内和细胞外生物合成抗菌纳米银颗粒
DOI：
10.1166/jnn.2017.13920
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发表时间：
2017-12
影响因子：
--
通讯作者：
Xuemei Wang
中科院分区：
文献类型：
--
作者：
Shuihong Li;Yuanrun Duan;Ranhui Li;Xuemei Wang
本研究提出了两种从细菌菌株铜绿假单胞菌CI109通过生物还原银离子制备银纳米颗粒(AgNPs)的微生物方法。银纳米粒对多药耐药(MDR)临床病原菌的抗菌效果也得到证实。用紫外可见光谱(UV-Vis)、扫描电子显微镜(SEM)、能量色散X射线能谱(EDX)、透射电子显微镜(TEM)和激光粒度分析仪对所合成的AgNPs进行了表征。此外，通过琼脂纸片扩散试验研究了所合成的AgNPs对多药耐药菌株的抗菌特性。UV分析表明，从12h到72h，两种生物合成方法合成的银纳米粒子的直径逐渐增大，其特征吸收峰随时间的推移表现出赤色效应。EDX表明了两种银纳米粒子在定域元素信息上的区别。以铜绿假单胞菌胞外液为还原剂(Av.14 nm)大于铜绿假单胞菌(Av.6 nm)。两种银纳米粒子对临床多药耐药病原菌均表现出良好的抗菌效果，且相关的抗菌性能表现出剂量依赖性。
This research work presents two microbial synthesis approaches of silver nanoparticles (AgNPs) from the bacterial strain Pseudomonas aeruginosa CI109 through bio-reduction of silver ions. The antibacterial efficacy of the AgNPs on multi-drug resistant (MDR) clinical pathogens was also confirmed. The synthesized AgNPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscope (TEM) and laser particle size analyzer. Furthermore, the antibacterial characteristics of the synthesized AgNPs against MDR strains were investigated by an agar disk diffusion test. The UV analysis demonstrated that the diameters of the synthetic silver nanoparticles using the two biosynthetic methods increased gradually from 12 h to 72 h as its characteristic absorption peaks showed a bathochromic effect with elapsed time. EDX indicated the distinctions of the two types of the silver nanoparticles in the localized elemental information. By SEM and TEM, synthetic silver nanoparticles were found to be spherical or ellipsoidal in shape and their diameter using the extracellular solution of P. aeruginosa as a reducing agent (Av. 14 nm) was larger than when the P. aeruginosa cells (Av. 6 nm) was used. The two types of the silver nanoparticles showed good antibacterial effects against clinical MDR pathogens, and their relevant antimicrobial properties demonstrated a dose-dependent manner.