A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice
A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice
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DOI：
10.1128/iai.71.9.4857-4861.2003
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发表时间：
2003-09-01
影响因子：
3.1
通讯作者：
Andrews, E
中科院分区：
文献类型：
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作者：
Oñate, AA;Céspedes, S;Andrews, E
本研究旨在评价牛布氏杆菌铜锌超氧化物歧化酶（Cu，Zn SOD）基因DNA疫苗的免疫原性和保护效果。将携带SOD基因的质粒DNA（pcDNA-SOD）肌肉注射BALB/c小鼠，可引起体液免疫和细胞免疫应答。动物注射pcDNA-SOD开发SOD特异性抗体表现出优势的免疫球蛋白G2 a（IgG 2a）超过IgG 1。此外，DNA疫苗引起T细胞增殖反应，也诱导γ干扰素的产生，但不是白细胞介素-10（IL-10）或IL-4，重组超氧化物歧化酶或粗布鲁氏菌蛋白再刺激后，表明诱导一个典型的T辅助细胞-1-主导的免疫反应在小鼠中。pcDNA-SOD（而非对照载体）在BALB/c小鼠中诱导针对B攻击的强的、显著水平的保护。牛流产杆菌强毒株2308的保护作用与B诱导的保护作用相似。流产疫苗株RB 51。总的来说，这些数据表明，pcDNA-SOD是一个很好的候选人，用于未来的研究接种布鲁氏菌病。
This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.