Extensive translational regulation during seed germination revealed by polysomal profiling.
Extensive translational regulation during seed germination revealed by polysomal profiling.
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DOI：
10.1111/nph.14355
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Hanson J
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文献类型：
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作者：
Bai B;Peviani A;van der Horst S;Gamm M;Snel B;Bentsink L;Hanson J
这项工作调查的翻译调节在种子萌发过程中的程度。通过总mRNA和多核糖体相关mRNA的全基因组谱确定每个基因的多核糖体占有率。这揭示了拟南芥种子萌发过程中广泛的翻译调控。在萌发过程中，数千个单独的mRNA的多核糖体占据在很大程度上发生变化。有趣的是，这些变化仅限于两个时间阶段（移位）在发芽，种子水合和发芽。序列特征，如上游开放阅读框数、转录物长度、mRNA稳定性、二级结构以及与该翻译调控相关的特定基序的存在和位置。这些功能之间的差异显着的两个转变，表明独立的机制调节种子萌发过程中的翻译。这项研究揭示了种子萌发过程中大量的翻译动力学，并确定了与翻译控制相关的发育依赖性序列特征和顺式元件，揭示了种子萌发过程中基因调控的一个新的重要层面。
This work investigates the extent of translational regulation during seed germination. The polysome occupancy of each gene is determined by genome‐wide profiling of total mRNA and polysome‐associated mRNA. This reveals extensive translational regulation during Arabidopsis thaliana seed germination. The polysome occupancy of thousands of individual mRNAs changes to a large extent during the germination process. Intriguingly, these changes are restricted to two temporal phases (shifts) during germination, seed hydration and germination. Sequence features, such as upstream open reading frame number, transcript length, mRNA stability, secondary structures, and the presence and location of specific motifs correlated with this translational regulation. These features differed significantly between the two shifts, indicating that independent mechanisms regulate translation during seed germination. This study reveals substantial translational dynamics during seed germination and identifies development‐dependent sequence features and cis elements that correlate with the translation control, uncovering a novel and important layer of gene regulation during seed germination.