Identification of candidate methylation-responsive genes in ovarian cancer.
Identification of candidate methylation-responsive genes in ovarian cancer.
复制标题
在卵巢癌中鉴定候选甲基化响应基因。
DOI：
10.1186/1476-4598-6-10
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发表时间：
2007-01-25
期刊：
影响因子：
37.3
通讯作者：
McDonald, John F.
中科院分区：
文献类型：
--
作者：
Menendez, Laura;Walker, DeEtte;Matyunina, Lilya V.;Dickerson, Erin B.;Bowen, Nathan J.;Polavarapu, Nalini;Benigno, Benedict B.;McDonald, John F.
基因启动子区域的异常甲基化与癌症发展和进展中基因表达的变化有关。与CpG岛（CGI）相关的基因特别容易发生甲基化，但并非所有CGI相关基因在癌症中都会显示甲基化模式的变化。为了鉴定受甲基化调控的基因，我们对卵巢癌细胞系（OVCAR-3）在用去甲基化剂5-氮杂脱氧胞苷（5-aza-dC）处理前后进行了基因表达谱分析。这些基因的重叠子集被发现显示正常卵巢表面上皮细胞和卵巢癌分离的恶性细胞之间的基因表达的显着差异。虽然所有人类基因的40%与CGI相关，但> 94%的重叠基因子集与CGI相关。实验验证了从重叠子集中随机选择的基因的甲基化状态的预测变化。我们的结论是，相关的基因上调5-aza-dC治疗的癌细胞系与基因下调的癌细胞可能是一个有用的方法，以确定基因经历表观遗传介导的变化，在癌症的发展表达。
Aberrant methylation of gene promoter regions has been linked to changes in gene expression in cancer development and progression. Genes associated with CpG islands (CGIs) are especially prone to methylation, but not all CGI-associated genes display changes in methylation patterns in cancers. In order to identify genes subject to regulation by methylation, we conducted gene expression profile analyses of an ovarian cancer cell line (OVCAR-3) before and after treatment with the demethylating agent 5-aza-deoxycytidine (5-aza-dC). An overlapping subset of these genes was found to display significant differences in gene expression between normal ovarian surface epithelial cells and malignant cells isolated from ovarian carcinomas. While 40% of all human genes are associated with CGIs, > 94% of the overlapping subset of genes is associated with CGIs. The predicted change in methylation status of genes randomly selected from the overlapping subset was experimentally verified. We conclude that correlating genes that are upregulated in response to 5-aza-dC treatment of cancer cell lines with genes that are down-regulated in cancer cells may be a useful method to identify genes experiencing epigenetic-mediated changes in expression over cancer development.
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影响因子：
4.4
作者：
Dannenberg, Luke O;Edenberg, Howard J
通讯作者：
Edenberg, Howard J
影响因子：
6.4
作者：
Wang, ZQ;Zhang, J;Lim, SH
通讯作者：
Lim, SH
DOI：
10.1073/pnas.091062498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Tusher, VG;Tibshirani, R;Chu, G
通讯作者：
Chu, G
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作者：
Cameron, EE;Bachman, KE;Baylin, SB
通讯作者：
Baylin, SB
影响因子：
37.3
作者：
Ducasse M;Brown MA
通讯作者：
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