Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity
Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity
复制标题
通过高覆盖率单细胞 RNA 测序和功能异质性鉴定体感神经元类型
DOI：
10.1038/cr.2015.149
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发表时间：
2016-01-01
期刊：
影响因子：
44.1
通讯作者：
Zhang, Xu
中科院分区：
文献类型：
--
作者：
Li, Chang-Lin;Li, Kai-Cheng;Zhang, Xu
感觉神经元通过不同的信号网络和接受特性来区分。因此，感觉神经元类型可以通过将基于转录组的神经元分型与感觉表型相关联来定义。在这里，我们分类小鼠背根神经节（DRG）的体感神经元的高覆盖率单细胞RNA测序（10 950 +/-1 218个基因每个神经元）和神经元大小为基础的层次聚类。此外，单个DRG神经元对皮肤刺激的反应使用体内全细胞膜片钳技术记录，并通过神经元类型遗传标记进行分类。小直径DRG神经元分为一类低阈值机械感受器和五类机械热伤害感受器（MHN）。每种MHN类型进一步分为两个亚型。大的DRG神经元分为四种类型，包括neurexophilin 1表达MHN和机械伤害感受器（MN）表达BAI1相关蛋白2样1（Baiap2l1）。表达运输蛋白颗粒复合物3样和Baiap2l1标记的MN的机械感受器各自细分为两种亚型。这些结果为体感神经元及其转录组数据库的编目提供了一个新的系统。
Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 +/- 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.