Genetic diversity and variation over time of Coxiella burnetii genotypes in dairy cattle and the farm environment
Genetic diversity and variation over time of Coxiella burnetii genotypes in dairy cattle and the farm environment
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DOI：
10.1016/j.meegid.2015.02.006
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发表时间：
2015-04-01
影响因子：
3.2
通讯作者：
Hurtado, Ana
中科院分区：
文献类型：
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作者：
Pinero, Alvaro;Barandika, Jesus F.;Hurtado, Ana
通过多位点可变数量串联重复分析（MLVA）测定了36个奶牛群的布美柯克斯体的遗传多样性，并对不同来源（散装奶-BTM和表面灰尘）和采样时间（2009110和2011/12）的基因型进行了比较。从 60 个 BTM 和 7 个灰尘样本中总共鉴定出 15 种不同的基因型，其中 7 种基因型是首次报告的（BN、BO、BP、BQ BR、BS、BT）。在 BTM 和灰尘中均检测到的两种最常见的基因型（J 和 I）占布美柯克斯体分型的 44.5%，据报道在全世界感染牛。在 52% 的牛群中发现了不止一种基因型，并且在 7 个 BTM 样本中观察到两种基因型的混合感染。对每个牛群内不同样本的布美柯克斯体基因型进行比较，发现 32% 的牛群中基因型发生变化，而其余 68% 的牛群中发现了持续的基因型。此外，从灰尘样本中获得的基因型始终与 BTM 样本中的基因型相同。通常，持久性基因型是最普遍的类型之一。使用最小生成树方法对本研究和其他研究中的 MLVA 基因型进行聚类，将布美柯克斯体菌株分成两个簇，其中 10 个基因型聚类在基因组组 (GG) III 中，其余 5 种类型（AE、BQ BR、BS 和 BT）归入 GG II 组，其中包括与人类疫情有关的菌株。尽管牛中存在与人类密切相关的基因型似乎并不常见，但它不能被忽视，需要监测基因型分布以充分了解 Q 热的流行病学。 (C) 2015 Elsevier B.V. 保留所有权利。
The genetic diversity of Coxiella bumetii from 36 dairy cattle herds was determined by Multiple-Locus Variable number tandem repeats Analysis (MLVA), and genotypes from different sources (bulk-tank milk - BTM and surface dust) and sampling time (2009110 and 2011/12) were compared. A total of 15 different genotypes were identified from 60 BTM and seven dust samples, including seven genotypes reported here for the first time (BN, BO, BP, BQ BR, BS, BT). The two most prevalent genotypes (J and I), detected both in BTM and dust, accounted for 44.5% of the Coxiella bumetii typed and have been reported infecting cattle worldwide. In 52% of herds more than one genotype was found, and mixed infection with two genotypes was observed in seven BTM samples. Comparison of Coxiella bumetii genotypes at different samplings within each herd detected a change in genotype in 32% of herds, while a persistent genotype was identified in the remaining 68%. In addition, the genotype obtained from dust samples was always identical to that present in the BTM sample. Often persistent genotypes were among the most prevalent types. Clustering of the MLVA genotypes from this and other studies using the minimum spanning tree method separated our Coxiella bumetii strains into two clusters, 10 genotypes clustered within genomic group (GG) III, and the remaining five types (AE, BQ BR, BS and BT) grouped with GG II, which includes strains implicated in human outbreaks. Although presence in cattle of genotypes closely related to those identified in humans does not seem to be common event, it cannot be neglected and surveillance of genotype distribution is needed to fully understand the epidemiology of Q fever. (C) 2015 Elsevier B.V. All rights reserved.