Chromosome instability and unusual features of some widely used strains of Candida albicans
Chromosome instability and unusual features of some widely used strains of Candida albicans
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DOI：
10.1002/yea.1597
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发表时间：
2008-06-01
期刊：
影响因子：
2.6
通讯作者：
Rustchenko, Elena
中科院分区：
文献类型：
--
作者：
Ahmad, Ausaf;Kabir, M. Anaul;Rustchenko, Elena
对白色念珠菌进行的核型分析显示，在测序菌株SC5314的三个不同种群中，ChR具有不同的迁移模式。在一个种群中，CBR大小的高度不稳定性阻止了CHR作为致密带的迁移，而CBR则以涂抹的形式出现。在一些群体中，Chr和/或Ch1倍体减少，表明二体和单体细胞的混合群体。同样，一些广泛使用的衍生品CAI4和BWP17的库存中也含有CHR污点。此外，在SC5314的谱系中，最受操纵的菌株是最后一个衍生物BWP17，它获得了CH7b大小的增加，并显示了一种不同寻常的特性。BWP17不能容忍成熟的端粒介导的染色体碎裂过程；剩下的完整同源物总是重复的。我们认为，SC5314的一些股票不稳定，BWP17可能不适合进行一般研究。我们建议将CAF4-2用于一般研究，而不是BWP17或CAI4，CAF4-2是一种相对稳定的Ura(-)衍生物，在我们实验室已成功使用了十多年。版权所有(D 2008 John Wiley父子，Ltd.
Electrophoretic karyotyping of the Candida albicans revealed a different migration pattern of ChR in three different stocks of the sequencing strain SC5314. In one stock, the high instability of CbR size prevented the migration of ChR as a compact band; CbR appeared, instead, as a smear. In some stocks, ChR and/or Ch1 ploidy diminished, suggesting mixed populations of disomic and monosomic cells. Similarly, some stocks of widely used derivatives CAI4 and BWP17 contained smearing of ChR. In addition, the most manipulated strain in the lineage of SC5314, the last derivative, BWP17, acquired an increase in the size of Ch7b and revealed an unusual property. BWP17 did not tolerate a well-established procedure of telomere-mediated fragmentation of a chromosome; the remaining intact homologue always duplicated. We suggest that some stocks of SC5314 are unstable and that BWP17 may not be appropriate for general studies. Instead of BWP17 or CAI4, we recommend using for general research CAF4-2, which is a relatively stable Ura(-) derivative, and which has been successfully used for more than a decade in our laboratory. Copyright (D 2008 John Wiley & Sons, Ltd.