Preparation of goat milk anti-Saccharomyces cerevisiae immunoglobulin G (IgG)-rich fraction and immunological functions of mice orally administered a mixture of the antigen and its specific IgG-rich fraction
Preparation of goat milk anti-Saccharomyces cerevisiae immunoglobulin G (IgG)-rich fraction and immunological functions of mice orally administered a mixture of the antigen and its specific IgG-rich fraction
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发表时间：
2009
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通讯作者：
Yumi Sekimura;N. Onodera;Minami Banno;I. Hata;K. Hamano;T. Shimosato;H. Otani
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作者：
Yumi Sekimura;N. Onodera;Minami Banno;I. Hata;K. Hamano;T. Shimosato;H. Otani
本研究对酵母菌Saccharomyces（S.）从山羊乳中制备的酵母菌特异性免疫球蛋白G-富集组分（抗酵母IgG）对小鼠免疫系统的影响。实时荧光定量PCR分析表明，在单独使用抗酵母IgG或与S.酿酒酵母和抗酵母IgG比在与S.酿酒酵母单独。相反，与S.酿酒酵母单独，虽然这是高于单独的IgG。因此，5周龄C3 H/HeN小鼠口服施用生理盐水（对照）组或S.实验组每天1次，连续5周。我们发现给予供试品溶液的小鼠血清总IgG水平显著低于给予对照溶液的小鼠。从小鼠派伊尔集合淋巴结细胞提取的mRNA的微阵列分析显示，与B细胞增殖和分化、T细胞活化和Th 2细胞分化相关的基因的表达谱在实验组中低于对照组。相反，与调节性T细胞相关的基因在给予试验溶液的小鼠中更高地表达。此外，发现口服给药供试品溶液可减轻由螨抗原诱导的NC/Nga小鼠的过敏症状。与对照组相比，试验小鼠脾脏白细胞介素-4 + CD 4+细胞数量减少。这些结果表明，口服S.酿酒酵母及其特异性山羊乳IgG富集组分可抑制小鼠I型变态反应症状的发展。
In the current study, we investigated the eŠects of Saccharomyces (S.) cerevisiae and its speciˆc immunoglobulin G rich fraction (anti-yeast IgG) prepared from goat's milk on the mouse immune system. Real-time polymerase chain reaction (PCR) analysis of mRNA extracted from C3H/HeN mouse Peyer's patch cells revealed that the expression of Rnf128 on regulatory T cells was higher in the cells cultured with anti-yeast IgG alone or with a mixture of S. cerevisiae and anti-yeast IgG than in the cells cultured with S. cerevisiae alone. In contrast, the expression of Stat6 related to polarize type 2 helper T (Th2) cells was lower in the cells cultured with the mixture than S. cerevisiae alone, although that was higher than IgG alone. Hence, 5week-old C3H/HeN mice were orally administered with either saline solution (control) group or a mixture of S. cerevisiae and anti-yeast IgG in saline (test) group once a day for 5 weeks. We found total serum IgG levels to be signiˆcantly lower in mice administered the test solution than those that were given the control solution. Microarray analysis of mRNA extracted from the mouse Peyer's patch cells revealed that the expression proˆle of genes related to proliferation and diŠerentiation of B cells, T cell activation and diŠerentiation of Th2 cells was lower in the test mice than in the control group. In contrast, the genes related to regulatory T cells were more highly expressed in mice administered with the test solution. Moreover, oral administration of the test solution was found to reduce allergic symptoms in NC/Nga mice induced with a mite antigen. The number of spleen interleukin-4+CD4+ cells was reduced in test mice when compared to the control group. These ˆndings indicate that oral administration of the mixture of S. cerevisiae and its speciˆc goat milk IgGrich fraction may suppress the development of type I allergic symptoms in mice.