Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients
Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients
复制标题
胃肠道癌症患者中小隐孢子虫的患病率和基因分型
DOI：
10.7150/jca.42393
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发表时间：
2020-03
影响因子：
3.9
通讯作者：
Xichen Zhang
中科院分区：
文献类型：
--
作者：
Nan Zhang;Xiuyan Yu;Hongbo Zhang;Lianying Cui;Xiaoou Li;Xiaowei Zhang;Pengtao Gong;Jianhua Li;Ziyi Li;Xiaocen Wang;Xin Li;Ting Li;Xiaofeng Liu;Yanhui Yu;Xichen Zhang
胃肠道肿瘤是最常见的恶性肿瘤，占全球癌症发病率的1/5以上。越来越多的证据表明，隐孢子虫是一种顶端复合体原生动物，与胃肠道肿瘤密切相关。然而，隐孢子虫的流行。中国地区胃肠道癌症患者感染情况尚未统计。我们在此进行了一项病例对照研究，以评估隐孢子虫的发生情况。在化疗前的消化系统恶性肿瘤患者和对照人群中。用套式聚合酶链式反应扩增18S rRNA基因检测隐孢子虫的存在。在每个粪便样本中。这一结果证实了隐孢子虫的相关性。结直肠癌和肝癌的感染，而首次发现了高频率的隐孢子虫。在食道癌和小肠癌中。隐孢子虫的感染率。结直肠癌、食道癌、肝癌和小肠癌分别为17.24%(20/116，P<；0.001)、6.25%(1/16，P=0.029)、14.29%(1/7，P<；0.001)和40%(2/5，P<；0.001)。此外，分子鉴定表明，所有的隐孢子虫。获得的是微小隐孢子虫(C.parvum)，其18S rRNA序列与从牛中分离到的参考序列相同，表明可能存在人畜共患传播。此外，亚型分析表明，IIaA15G2R1和IIaA15G2R2是结直肠癌的主要亚型，而IIaA13G2R2亚型在结直肠癌和肝癌中首次命名和鉴定。首次将隐孢子虫的流行情况放在一起。据估计，中国人中有消化系统癌症患者的感染。我们的结果表明，微小隐孢子虫与胃肠道癌症有很高的相关性，支持隐孢子虫病可能是这些疾病的潜在危险因素。
Gastrointestinal cancers are the most commonly occurring malignancies which contributing to over 1/5 of cancer incidences worldwide. Increasing evidences have shown that Cryptosporidium spp., an apicomplexan protozoan, is highly associated with gastrointestinal cancers. However, the prevalence of Cryptosporidium spp. infections among gastrointestinal cancer patients in China has not been estimated yet. We here performed a case-control study to evaluate the occurrences of Cryptosporidium spp. in patients with digestive malignancies before chemotherapy and in control population. Nested PCR amplifying 18S rRNA gene was used to detect the presence of Cryptosporidium spp. in each fecal sample. The results herein confirmed the correlation of Cryptosporidium spp. infection with colorectal and liver cancers, while first identified the high frequencies of Cryptosporidium spp. in esophageal cancer and small intestine cancer. The infection rates of Cryptosporidium spp. in colorectal, esophageal, liver and small intestine cancers were 17.24% (20/116, P<0.001), 6.25% (1/16, P=0.029), 14.29% (1/7, P<0.001) and 40% (2/5, P<0.001), respectively. In addition, molecular characterization indicated that all the Cryptosporidium spp. obtained were Cryptosporidium parvum (C. parvum), and the 18S rRNA sequences were identical to the reference sequences isolated from cattle, suggesting potential zoonotic transmission. Furthermore, subtyping analyses revealed that IIaA15G2R1 and IIaA15G2R2 were the predominant subtypes in colorectal cancer, while IIaA13G2R2 subtype was first named and identified in colorectal and liver cancers. Taken together, for the first time, the prevalence of Cryptosporidium spp. infections in digestive cancer patients has been estimated among Chinese. Our results indicated that C. parvum were highly associated with gastrointestinal cancers, supporting that cryptosporidiosis could be a potential risk factor for these diseases.
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DOI：
10.3390/ijerph15091809
发表时间：
2018-08-22
影响因子：
--
作者：
Yang D;Yang Y;Wang Y;Yang Y;Dong S;Chen Y;Jiang Q;Zhou Y
通讯作者：
Zhou Y
影响因子：
3.2
作者：
Koehler AV;Korhonen PK;Hall RS;Young ND;Wang T;Haydon SR;Gasser RB
通讯作者：
Gasser RB
影响因子：
11.8
作者：
Feng Y;Wang L;Duan L;Gomez-Puerta LA;Zhang L;Zhao X;Hu J;Zhang N;Xiao L
通讯作者：
Xiao L
影响因子：
3.7
作者：
Benamrouz S;Guyot K;Gazzola S;Mouray A;Chassat T;Delaire B;Chabé M;Gosset P;Viscogliosi E;Dei-Cas E;Creusy C;Conseil V;Certad G
通讯作者：
Certad G
影响因子：
29.4
作者：
Chen, XM;Levine, SA;LaRusso, NF
通讯作者：
LaRusso, NF