Integrating Protein Homeostasis Strategies in Prokaryotes
Integrating Protein Homeostasis Strategies in Prokaryotes
复制标题
DOI：
10.1101/cshperspect.a004366
复制
发表时间：
2011-04-01
影响因子：
7.2
通讯作者：
Bukau, Bernd
中科院分区：
文献类型：
--
作者：
Mogk, Axel;Huber, Damon;Bukau, Bernd
细菌细胞经常暴露于其环境的剧烈波动中，这导致蛋白质稳态的扰动并导致蛋白质错误折叠。因此，细菌进化出了强大的质量控制网络，由伴侣蛋白和蛋白酶组成，它们合作监测蛋白质的折叠状态，并通过重折叠或降解去除错误折叠的构象。质量控制组分的水平通过诱导室特异性应激反应调整到细胞蛋白质组的折叠状态。此外，几个质量控制组件的活动直接由这些应力控制，允许快速激活。然而，严重的压力可以克服蛋白质静止网络的保护功能，导致蛋白质聚集体的形成，这些聚集体被隔离在细胞极点。蛋白质聚集体要么被AAA+伴侣溶解，要么通过细胞分裂消除，从而产生无损伤的子细胞。
Bacterial cells are frequently exposed to dramatic fluctuations in their environment, which cause perturbation in protein homeostasis and lead to protein misfolding. Bacteria have therefore evolved powerful quality control networks consisting of chaperones and proteases that cooperate to monitor the folding states of proteins and to remove misfolded conformers through either refolding or degradation. The levels of the quality control components are adjusted to the folding state of the cellular proteome through the induction of compartment specific stress responses. In addition, the activities of several quality control components are directly controlled by these stresses, allowing for fast activation. Severe stress can, however, overcome the protective function of the proteostasis network leading to the formation of protein aggregates, which are sequestered at the cell poles. Protein aggregates are either solubilized by AAA+ chaperones or eliminated through cell division, allowing for the generation of damage-free daughter cells.