Induction of host defence responses by Drosophila C virus
Induction of host defence responses by Drosophila C virus
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DOI：
10.1099/vir.0.83684-0
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发表时间：
2008-06-01
影响因子：
3.8
通讯作者：
Johnson, Karyn N.
中科院分区：
文献类型：
--
作者：
Hedges, Lauren M.;Johnson, Karyn N.
昆虫对病毒感染的特异性反应已经用遗传学上易处理的黑腹果蝇进行了研究。大多数研究集中在与果蝇C病毒（DCV）的相互作用，DCV是双链病毒科的成员。DCV是一种无包膜、T=3二十面体病毒，具有正义RNA基因组。最近证明，由Jak-STAT途径控制的几个基因在DCV感染后特异性上调。为了研究诱导这些反应的病毒因子，我们使用Jak-STAT调节基因作为报告基因。用非感染性DCV颗粒或双链RNA对果蝇进行的攻击并没有刺激抗病毒反应基因的显著上调。此外，有没有报告基因的上调与三种不同的DCV菌株的果蝇之间的差异。这表明这些果蝇基因的上调可能需要病毒复制，并可能涉及DCV的非结构蛋白。
Insect responses that are specific for virus infection have been investigated using the genetically tractable Drosophila melanogaster. Most studies focus on interactions with Drosophila C virus (DCV), which is a member of the family Dicistroviridae. DCV is a non-enveloped, T=3 icosahedral virus with a positive-sense RNA genome. It was demonstrated recently that several genes controlled by the Jak-STAT pathway are specifically upregulated upon DCV infection. To investigate the virus factors that induce these responses, we used the Jak-STAT regulated genes as reporter genes. Challenge of flies with non-infectious DCV particles or double-stranded RNA did not stimulate significant upregulation of the antiviral response genes. In addition, there was no difference in reporter gene upregulation between Drosophila challenged with three different strains of DCV. This suggests that upregulation of these Drosophila genes may require virus replication and may involve the non-structural proteins of DCV.