Identification and characterization of HAK/KUP/KT potassium transporter gene family in barley and their expression under abiotic stress.
Identification and characterization of HAK/KUP/KT potassium transporter gene family in barley and their expression under abiotic stress.
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DOI：
10.1186/s12864-021-07633-y
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发表时间：
2021-05-01
期刊：
影响因子：
4.4
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Cai K;Zeng F;Wang J;Zhang G
HAK/KUP/KT(高亲和力K+转运体/K+吸收转运体/K+转运体)是植物中最大的钾转运体家族，在K+的吸收和运输以及生物和非生物胁迫反应中起着关键作用。然而，我们对大麦基因家族的认识还不够深入。是相当有限的。在本研究中，我们通过全基因组分析鉴定了27个大麦HAK/KUP/KT基因(以下简称HvHAKs)。这些HvHAK在7条染色体上分布不均，在系统发育上可分为4个类群。所有的HvHAK蛋白序列都含有保守的基序和结构域。然而，HAK成员在顺式作用元件和组织表达模式上存在实质性差异。小麦与大麦HAKS的同源基因最多，其次是短柄青霉、水稻和玉米。此外，利用qRT-PCR技术研究了6个大麦Hak基因在3种非生物胁迫下的表达谱，发现在盐胁迫、高渗胁迫和缺钾处理下，Hak基因的表达水平均上调。在大麦中已鉴定出27个Hak基因(HvHAKs)，它们在组织表达模式和对盐胁迫、干旱胁迫和缺钾的响应上存在差异。网上版载有补充材料，可在10.1186/s12864-021-07633-y查阅。
HAK/KUP/KT (High-affinity K+ transporters/K+ uptake permeases/K+ transporters) is the largest potassium transporter family in plants, and plays pivotal roles in K+ uptake and transport, as well as biotic and abiotic stress responses. However, our understanding of the gene family in barley (Hordeum vulgare L.) is quite limited. In the present study, we identified 27 barley HAK/KUP/KT genes (hereafter called HvHAKs) through a genome-wide analysis. These HvHAKs were unevenly distributed on seven chromosomes, and could be phylogenetically classified into four clusters. All HvHAK protein sequences possessed the conserved motifs and domains. However, the substantial difference existed among HAK members in cis-acting elements and tissue expression patterns. Wheat had the most orthologous genes to barley HAKs, followed by Brachypodium distachyon, rice and maize. In addition, six barley HAK genes were selected to investigate their expression profiling in response to three abiotic stresses by qRT-PCR, and their expression levels were all up-regulated under salt, hyperosmotic and potassium deficiency treatments. Twenty seven HAK genes (HvHAKs) were identified in barley, and they differ in tissue expression patterns and responses to salt stress, drought stress and potassium deficiency. The online version contains supplementary material available at 10.1186/s12864-021-07633-y.
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影响因子：
7.4
作者：
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通讯作者：
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