Human Hertwig's epithelial root sheath cells play crucial roles in cementum formation
Human Hertwig's epithelial root sheath cells play crucial roles in cementum formation
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DOI：
10.1177/154405910708600703
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发表时间：
2007-07-01
影响因子：
7.6
通讯作者：
Shi, S.
中科院分区：
文献类型：
--
作者：
Sonoyama, W.;Seo, B.-M.;Shi, S.
赫特维希上皮根鞘 (HERS) 细胞是牙周膜室中独特的上皮细胞群。迄今为止，它们的功能作用尚未完全阐明。我们的假设是，HERS 细胞可能参与调节牙周膜干细胞 (PDLSC) 的分化和体内牙骨质的形成。在这项研究中，我们发现HERS细胞可能能够在体外促进PDLSC分化并经历上皮间质转化。使用免疫组织化学染色、蛋白质印迹分析、transwell共培养系统和体内移植来表征HERS细胞和PDLSC之间的相互作用，以及HERS细胞的上皮间质转化（EMT）。 TGF beta 1能够通过激活PI3K/AKT途径诱导HERS细胞的上皮-间质转化。此外，当移植到免疫功能低下的小鼠体内时，HERS细胞能够形成牙骨质样组织。缩写：骨髓间充质干细胞，BMMSC；骨唾液蛋白，BSP；羟基磷灰石/磷酸三钙，HA/TCP；赫特维希上皮根鞘，HERS；骨钙素，OCN；牙周膜，PDL；牙周膜干细胞，PDLSC；磷脂酰肌醇 3-激酶，PI3K。
Hertwig's epithelial root sheath ( HERS) cells are a unique population of epithelial cells in the periodontal ligament compartment. To date, their functional role has not been fully elucidated. Our hypothesis was that HERS cells may be involved in regulating differentiation of periodontal ligament stem cells (PDLSCs) and forming cementum in vivo. In this study, we found that HERS cells may be capable of promoting PDLSC differentiation and undergoing epithelial-mesenchymal transition in vitro. Immunohistochemical staining, Western blot analysis, a transwell co-culture system, and in vivo transplantation were used to characterize the interplay between HERS cells and PDLSCs, as well as the epithelial-mesenchymal transition (EMT) of HERS cells. TGF beta 1 was capable of inducing the epithelial-mesenchymal transition of HERS cells through activating the PI3K/AKT pathway. Furthermore, HERS cells were able to form cementum-like tissue when transplanted into immunocompromised mice. Abbreviations: bone marrow mesenchymal stem cell, BMMSC; bone sialoprotein, BSP; hydroxyapatite/ tricalcium phosphate, HA/TCP; Hertwig's epithelial root sheath, HERS; osteocalcin, OCN; periodontal ligament, PDL; periodontal ligament stem cell, PDLSC; phosphatidylinositol 3-kinase, PI3K.