Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse
Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse
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DOI：
10.1111/j.1365-2443.2008.01210.x
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发表时间：
2008-08-01
期刊：
影响因子：
2.1
通讯作者：
Okabe, Masaru
中科院分区：
文献类型：
--
作者：
Yamaguchi, Ryo;Yamagata, Kazuo;Okabe, Masaru
CD52是一种糖基磷脂酰肌醇(GPI)锚定抗原，表达于淋巴细胞和附睾上皮细胞。CD52也被称为“成熟相关精子抗原”，但其功能尚不清楚。因此，我们产生了CD52干扰的小鼠。由此产生的CD52缺失的小鼠是健康的，尽管CD52在免疫系统的细胞上表达。然后我们研究了CD52在生殖中的可能作用。对CD52缺陷男性精子进行了研究，发现精子的存活率、活力、形态和自发性顶体反应的发生率都与野生型精子相似。在体外受精系统中，精子表现出正常的受精能力。由于CD52只有在移行到输精管后才被转移到精子上，我们研究了CD52缺陷小鼠的精子在体内的行为。小鼠自然交配，我们观察到正常数量的精子通过子宫输卵管交界处，这是导致不育精子的各种基因敲除的关键障碍。因此，野生型和CD52缺失型雄性的产仔数没有差异。因此，我们的结果表明，无论是在体内还是在体外受精，小鼠受精都不需要CD52。
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy, even though Cd52 is expressed on cells of the immune system. We then examined a possible role for CD52 in reproduction. Sperm from Cd52-deficient males were investigated and the viability, motility, morphology, and incidence of spontaneous acrosome reactions were found to be all similar to values for wild-type sperm. In in vitro fertilization system, the sperm showed normal fertilizing ability. As CD52 was found to be transferred onto sperm only after they had migrated into the vas deferens, we examined the behavior of sperm from Cd52-deficient mice in vivo. The mice mated naturally and we observed that a normal number of sperm passed through the uterotubal junction, known to the crucial hurdle for various gene knockouts resulting in infertile sperm. As a consequence, there was no difference in the litter size from the wild-type and Cd52-null males. Our results therefore indicate CD52 is not required for fertilization in the mouse either in vivo or in vitro.