Characterization of corrinoid compounds from a Japanese black tea (Batabata-cha) fermented by bacteria
Characterization of corrinoid compounds from a Japanese black tea (Batabata-cha) fermented by bacteria
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DOI：
10.1021/jf030585r
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发表时间：
2004-02-25
影响因子：
6.1
通讯作者：
Nakano, Y
中科院分区：
文献类型：
--
作者：
Kittaka-Katsura, H;Ebara, S;Nakano, Y
一种日本发酵红茶(Batabata-cha)含有相当数量的维生素B(12)(每100克干茶叶456+/-39 ng，每100毫升茶饮料2.0+/-0.3 ng)。从茶叶中分离得到一种皮质醇类化合物，对其进行了部分纯化和鉴定。当20周龄维生素B(12)缺乏的大鼠以尿中大量(约250 mg/天)的甲基丙二酸作为维生素B(12)缺乏的指标，给予茶饮料(50毫升/天，1 ng维生素B(12))6周后，经硅胶60薄层层析和C18反相高效液相色谱纯化的化合物与正品维生素B(12)的图谱相同。补充茶饮料的26周龄大鼠尿甲基丙二酸排泄量(169+/-29 mg/d)明显低于缺乏茶饮料的大鼠(250+/-32 mg/d)。结果表明，发酵红茶中发现的维生素B(12)在哺乳动物中是生物可利用的。
A Japanese fermented black tea (Batabata-cha) contained a considerable amount of vitamin B(12) (456 +/- 39 ng per 100 g dry tea leaves and 2.0 +/- 0.3 ng per 100 mL of tea drink). A corrinoid compound was partially purified and characterized from the tea leaves. The patterns of the purified compound by the silica gel 60 thin-layer chromatography and C18 reversed phased high-performance liquid chromatography were identical to those of authentic vitamin B(12), When 20 week old vitamin B(12) deficient rats, which excreted substantial amounts (about 250 mg/day) of methylmalonic acid in urine as an index of vitamin B(12) deficiency, were fed the tea drink (50 mL/day, 1 ng of vitamin B(12)) for 6 weeks, urinary methylmalonic acid excretion (169 +/- 29 mg/day) of the tea drink-supplemented 26 week old rats decreased significantly relative to that (250 +/- 32 mg/day) of the deficient rats. The results indicate that the vitamin B(12) found in the fermented black tea is bioavailable in mammals.