Two pivotal RNA editing sites in the mitochondrial atp1 mRNA are required for ATP synthase to produce sufficient ATP for cotton fiber cell elongation
Two pivotal RNA editing sites in the mitochondrial atp1 mRNA are required for ATP synthase to produce sufficient ATP for cotton fiber cell elongation
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ATP 合酶需要线粒体 atp1 mRNA 中的两个关键 RNA 编辑位点才能产生足够的 ATP 以供棉纤维细胞伸长
DOI：
10.1111/nph.14999
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发表时间：
2018-04-01
期刊：
影响因子：
9.4
通讯作者：
Yu, Jianing
中科院分区：
文献类型：
--
作者：
He, Peng;Xiao, Guanghui;Yu, Jianing
RNA编辑是影响陆地植物细胞器转录的转录后成熟过程。然而，RNA编辑的分子功能和生理作用仍然知之甚少。利用高通量测序技术，我们在陆地棉线粒体基因组中确定了692个RNA编辑位点。在编码区共有422个编辑位点，所有的编辑都是胞苷(C)到尿苷(U)的转化。比较分析表明，Ghatp1的两个编辑位点C1292和C1415的纤维编辑效率和胚珠编辑效率存在显著差异。生化和遗传分析表明，这两个重要的编辑位点对ATP合成酶和亚单位之间的相互作用具有重要作用，从而导致酵母中ATP的积累和促进细胞的生长。C1292、C1415或双重编辑的Ghatp1在拟南芥中的异位表达显著增加了叶片中的毛状体数量和根长。我们的结果表明，Ghatp1的C1292和C1415位的编辑对于ATP合成酶产生足够的ATP用于棉纤维细胞伸长至关重要。这项工作扩大了我们对ATP1和ATP合成中RNA编辑的理解，并为深入了解线粒体编辑的ATP1蛋白在高等植物中的功能提供了见解。
RNA editing is a post-transcriptional maturation process affecting organelle transcripts in land plants. However, the molecular functions and physiological roles of RNA editing are still poorly understood. Using high-throughput sequencing, we identified 692 RNA editing sites in the Gossypium hirsutum mitochondrial genome. A total of 422 editing sites were found in the coding regions and all the edits are cytidine (C) to uridine (U) conversions. Comparative analysis showed that two editing sites in Ghatp1, C1292 and C1415, had a prominent difference in editing efficiency between fiber and ovule. Biochemical and genetic analyses revealed that the two vital editing sites were important for the interaction between the and subunits of ATP synthase, which resulted in ATP accumulation and promoted cell growth in yeast. Ectopic expression of C1292, C1415, or doubly edited Ghatp1 in Arabidopsis caused a significant increase in the number of trichomes in leaves and root length. Our results indicate that editing at C1292 and C1415 sites in Ghatp1 is crucial for ATP synthase to produce sufficient ATP for cotton fiber cell elongation. This work extends our understanding of RNA editing in atp1 and ATP synthesis, and provides insights into the function of mitochondrial edited Atp1 protein in higher plants.