7-[(Chlorocarbonyl)methoxy]-4-methylcoumarin: a novel fluorescent reagent for the precolumn derivatization of hydroxy compounds in liquid chromatography.
7-[(Chlorocarbonyl)methoxy]-4-methylcoumarin: a novel fluorescent reagent for the precolumn derivatization of hydroxy compounds in liquid chromatography.
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7-[(氯羰基)甲氧基]-4-甲基香豆素：一种新型荧光试剂，用于液相色谱中羟基化合物的柱前衍生化。
DOI：
10.1021/ac00279a053
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发表时间：
1985
影响因子：
7.4
通讯作者：
Novotny,M
中科院分区：
文献类型：
--
作者：
Karlsson,KE;Wiesler,D;Alasandro,M;Novotny,M
本文报道了一种新试剂7-[（氯羰基）甲氧基]-4-甲基香豆素的合成及其荧光衍生物在液相色谱中的应用。以羟基甾体和洋地黄素为模型化合物，对反应条件进行了优化。高色谱效率的微柱被用来解决标准和生物混合物中的异构体化合物。
A new reagent, 7-[(chlorocarbonyl) methoxy]-4-methyl-coumarln, has been synthesized to form fluorescent derivatives for the liquid chromatography of various hydroxy com-pounds. Reaction conditions were optimized with model hy-droxy steroids and prostaglandins. Microcolumns of high chromatographic efficiency were employed to resolve Isom-eric compounds In both standard and biological mixtures.