Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing.
Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing.
复制标题
伤口愈合过程中兔体内角质形成细胞纤连蛋白受体功能的激活。
DOI：
10.1111/1523-1747.ep12355243
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Grinnell,F
中科院分区：
文献类型：
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作者：
Takashima,A;Billingham,RE;Grinnell,F
新鲜分离的兔角质形成细胞表达纤连蛋白 (pFN) 受体功能较低，其在 pFN 包被的基质上附着和扩散的能力较差，或者结合和摄取 pFN 包被的珠子的能力较差。然而，这些细胞体外培养后，pFN 受体功能被激活。培养的细胞似乎是正常的，因为它们能够快速使全层皮肤伤口床重新上皮。将新鲜分离的具有低 pFN 受体功能的角质形成细胞自体移植到全层伤口床上。移植两天后，观察到从这些伤口恢复的角质形成细胞表达增强的pFN受体功能。这种活性在移植后3天分离的角质形成细胞中达到最大，并且在随后分离的角质形成细胞中下降。移植后10天，移植细胞已形成多层增生表皮，并重建其层粘连蛋白和含IV型胶原的基底膜。有人提出，角质形成细胞中 pFN 受体功能的启动是角质形成细胞附着并迁移通过由肉芽组织组成的富含 pFN 的伤口床所必需的关键机制。上皮再生完成且基底膜重新形成后，pFN 受体功能显着下降，因为它不再是细胞所必需的。
Freshly isolated rabbit keratinocytes expressed low fibronectin (pFN) receptor function as shown by their poor ability to attach and spread on pFN-coated substrata or to bind and ingest pFN-coated beads. Following in vitro culture of these cells, however, pFN receptor function was activated. The cultured cells appeared to be normal, based on their ability to reepithelize rapidly full-thickness cutaneous wound beds. Freshly isolated keratinocytes that had low pFN receptor function were autotransplanted onto full-thickness wound beds. Two days after transplantation, keratinocytes recovered from these wounds were observed to express increased pFN receptor function. This activity was maximal in keratinocytes isolated 3 days after transplantation and declined in keratinocytes isolated at later times. By 10 days after transplantation, the transplanted cells had formed a multilayered hyperplastic epidermis and reconstituted their laminin and type IV collagen-containing basement membrane. It is proposed that initiation of pFN receptor function in keratinocytes is a crucial mechanism necessary for them to attach to and migrate through the pFN-rich wound bed comprised of granulation tissue. After reepithelization is complete, and the basement membrane re-forms, pFN receptor function declines markedly because it is no longer essential to the cells.