Production of an autoinducer of growth by norepinephrine cultured Escherichia coli O157:H7.
Production of an autoinducer of growth by norepinephrine cultured Escherichia coli O157:H7.
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DOI：
10.1111/j.1574-6968.1996.tb08196.x
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发表时间：
1996-06
影响因子：
2.1
通讯作者：
M. Lyte;C. Frank;B. Green
中科院分区：
文献类型：
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作者：
M. Lyte;C. Frank;B. Green
大肠杆菌O 157：H7在去甲肾上腺素的存在下培养以产生条件培养基。通过测量条件培养基支持大肠杆菌O 157：H7新鲜培养物生长的能力，检查条件培养基中是否存在生长诱导因子。与对照相比，用低至0.024%（v/v）的去甲肾上腺素条件培养基补充新鲜培养物导致生长增加，从而表明存在生长的自诱导物。在条件培养基生成期间对自诱导物的生产动力学的分析表明，其不同于其他更好表征的自诱导物。有人提出，宿主的神经体液环境可能有助于细菌生长因子的产生。
Escherichia coli O157:H7 were cultured in the presence of norepinephrine to generate conditioned media. The presence of a growth-inducing factor(s) in the conditioned media was examined by measurements of the ability of conditioned media to support the growth of fresh cultures of E coli O157:H7. Supplementation of fresh cultures with as little as 0.024% (v/v) norepinephrine conditioned medium resulted in increased growth as compared to controls, thereby indicating the presence of an autoinducer of growth. Analysis of the production kinetics for the autoinducer during the generation of conditioned media indicates that it differs from other more well characterized autoinducers. It is proposed that the neurohumoral environment of the host may contribute to the production of bacterial growth factors.