Aerobic and Anaerobic Ammonia-Oxidizing Microorganisms in Low-Temperature Hydrothermal Fe-Si-rich Precipitates of the Southwestern Pacific Ocean
Aerobic and Anaerobic Ammonia-Oxidizing Microorganisms in Low-Temperature Hydrothermal Fe-Si-rich Precipitates of the Southwestern Pacific Ocean
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DOI：
10.1080/01490451.2013.802397
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发表时间：
2014-01
影响因子：
2.3
通讯作者：
J. Li;Yannan Sun;Jiasong Fang;Weizhi Xie;Xiaotong Peng;Liang Dong;Zijun Wu-;Huaiyang Zhou
中科院分区：
文献类型：
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作者：
J. Li;Yannan Sun;Jiasong Fang;Weizhi Xie;Xiaotong Peng;Liang Dong;Zijun Wu-;Huaiyang Zhou
富Fe-Si热液沉淀物广泛分布于低温扩散热液场中。由于铁氧化细菌（FeOB）对这种类型的热液沉淀物的形成有重要贡献，以前的研究主要集中在研究FeOB相关的微生物种群，尽管这些沉淀物实际上容纳了丰富的其他微生物群落，特别是那些参与海洋氮循环的微生物群落。在这项研究中，我们调查的组成，多样性和丰富的好氧和厌氧氨氧化微生物居住在低温富铁硅热液沉淀物的劳综合研究基地的氨单加氧酶（amoA）基因和16 S rRNA基因的基础上。系统发育分析表明，富铁硅热液沉淀物中普遍存在氨氧化古菌（AOA）、亚硝化螺旋菌类氨氧化菌（AOB）和厌氧氨氧化厌氧氨氧化菌（细菌）。定量PCR分析表明，AOA在整个微生物群落中占优势，古细菌amoA基因丰度比AOB和厌氧氨氧化细菌高2-3个数量级。甘油二烷基甘油四醚分析结果证实AOA的存在和丰度。研究结果表明，微生物氨氧化，特别是古生菌好氧氨氧化，是低温扩散热液田中普遍存在的关键过程。本文提供了补充材料。去出版商的在线版地球微生物学杂志查看补充文件。
Fe-Si-rich hydrothermal precipitates are distributed widely in low-temperature diffusing hydrothermal fields. Due to the significant contribution of Fe-oxidizing bacteria (FeOB) to the formation of this type of hydrothermal precipitates, previous studies focus mostly on investigating FeOB-related microbial populations, albeit these precipitates actually accommodate abundant other microbial communities, particularly those involved in marine nitrogen cycle. In this study, we investigated the composition, diversity, and abundance of aerobic and anaerobic ammonia-oxidizing microorganisms dwelling in low-temperature Fe-Si-rich hydrothermal precipitates of the Lau Integrated Study Site based on ammonia monooxygenase (amoA) gene and 16S rRNA gene. Phylogenetic analysis revealed the common presence of ammonia-oxidizing archaea (AOA), Nitrosospira-like ammonia-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing anammox (bacteria) in the Fe-Si-rich hydrothermal precipitates. Quantitative PCR analysis showed that AOA dominated the whole microbial community and the abundance of archaeal amoA gene was 2–3 orders of magnitude higher than that of AOB and anammox bacteria. Result of glycerol dialkyl glycerol tetraether analysis confirmed the presence and abundance of AOA. Our results suggest that microbial ammonia oxidations, especially archaeal aerobic ammonia oxidation, are prevalent and pivotal processes in low-temperature diffusing hydrothermal fields. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the supplemental file.