High mobility group box 1 (HMGB1) and macrophage migration inhibitory factor (MIF) in Kawasaki disease
High mobility group box 1 (HMGB1) and macrophage migration inhibitory factor (MIF) in Kawasaki disease
复制标题
DOI：
10.1080/03009740802144143
复制
发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Hara, T.
中科院分区：
文献类型：
--
作者：
Hoshina, T.;Kusuhara, K.;Hara, T.
目的：探讨高迁移率族蛋白B1(HMGB1)和巨噬细胞移动抑制因子(MIF)两种促炎细胞因子在川崎病(KD)发病中的作用。11例脓毒症患儿和28例健康儿童作为对照。用相应的酶联免疫吸附试验(ELISA)试剂盒检测血清HMGB1和MIF水平。采用实时定量聚合酶链式反应(RT-PCR)技术检测外周血单个核细胞(PBMC)中晚期糖基化终产物受体(RAGE)、MIF受体CD74和HMGB1受体基因的表达水平。KD患者血清HMGB1和MIF水平显著高于对照组(P
Objective: To investigate whether two proinflammatory cytokines, high mobility group box 1 (HMGB1) and macrophage migration inhibitory factor (MIF) are involved in the development of Kawasaki disease (KD).Methods: Twenty-seven patients with KD were included in this study. Eleven patients with sepsis and 28 healthy children served as controls. Serum levels of HMGB1 and MIF were measured by corresponding enzyme-linked immunosorbent assay (ELISA) kits, respectively. Real-time polymerase chain reaction (PCR) was used to quantify the expression levels of genes encoding receptor for advanced glycation end-products (RAGE), an HMGB1 receptor, and CD74, an MIF receptor in peripheral blood mononuclear cells (PBMCs).Results: Serum levels of HMGB1 and MIF in KD patients were the highest in the early acute phase and gradually decreased after defervescence. Serum HMGB1 and MIF levels in KD patients were significantly higher than those in controls (HMGB1, p