Search for Candidate Genes Causing the Excessive Ca Accumulation in Roots of Tipburn-Damaged Lisianthus (Eustoma grandiflorum) Cultivars
Search for Candidate Genes Causing the Excessive Ca Accumulation in Roots of Tipburn-Damaged Lisianthus (Eustoma grandiflorum) Cultivars
复制标题
DOI：
10.3390/agriculture11030254
复制
发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Takanori Kuronuma;Hitoshi Watanabe
中科院分区：
文献类型：
--
作者：
Takanori Kuronuma;Hitoshi Watanabe
摘要烧尖病是影响洋桔梗生产的一个严重问题。以往的研究表明，在根中的钙烧伤损害品种，在那里的钙分配到顶端叶尖的抑制过量积累。然而，很少有研究调查钙积累和园艺作物基因表达之间的关联。为了提供可能导致根中过量Ca积累的候选靶基因的列表，我们集中于发生梢枯的品种（“Voyage peach”：VP）和不发生梢枯的品种（“Umi honoka”：UH）的上部叶和根的Ca 2+转运蛋白和果胶甲基酯酶（PME）基因和RNA-seq。在VP的上部叶和根中，编码谷氨酸受体（GLR）、阳离子/Ca 2+交换体4（CCX 4）、Na+/Ca 2+交换体样蛋白（NCL）和PME的基因上调，编码环核苷酸门控离子通道9（CNGC 9）的基因下调。相反，编码液泡阳离子/质子交换体5（CAX 5）、钙转运ATP酶1和12（ACA 1和ACA 12）的基因在每个器官中显示出差异表达。其中，只有CAX 5在VP根部表达上调，而ACA 12在VP根部表达下调。因此，我们认为CAX 5和ACA 12是引起烧尖型洋桔梗根系钙积累过多的候选基因。未来的研究应利用定量PCR技术研究烧尖损伤后洋桔梗品种基因表达的时间变化，并对候选基因进行功能分析。
Occurrence of tipburn is a severe problem in the production of lisianthus cultivars. Previous studies have shown excessive Ca accumulation in the roots of tipburn-damaged cultivars, where the distribution of Ca to the tips of the top leaves is inhibited. However, few studies have investigated the association between Ca accumulation and gene expression in horticultural crops. To provide a list of candidate target genes that might be causing the excessive Ca accumulation in roots, we focused Ca2+ transporter and pectin methylesterase (PME) genes and RNA-seq of upper leaves and roots in tipburn-occurrence cultivar (“Voyage peach”: VP) and non-occurrence cultivar (“Umi honoka”: UH) was conducted. In both the upper leaves and roots of VP, genes encoding the glutamate receptors (GLRs), cation/Ca2+ exchangers 4 (CCX4), Na+/Ca2+ exchanger-like protein (NCL), and PMEs were upregulated, and a gene encoding the cyclic nucleotide-gated ion channel 9 (CNGC9) was downregulated. In contrast, genes encoding the vacuolar cation/proton exchanger 5 (CAX5), calcium-transporting ATPase 1 and 12 (ACA1 and ACA12) showed differential expression in each organ. Among them, only CAX5 was upregulated and ACA12 was downregulated in the roots of VP. Based on these results, we suggested that CAX5 and ACA12 are the candidate genes causing the excessive Ca accumulation in the roots of tipburn-occurrence lisianthus cultivars. Future studies should investigate the temporal changes in gene expression using quantitative PCR and conduct functional analysis of candidate genes in tipburn-damaged lisianthus cultivars.