ESSENTIAL ROLE OF NF-KAPPA-B ACTIVATION IN SILICA-INDUCED INFLAMMATORY MEDIATOR PRODUCTION IN MACROPHAGES
ESSENTIAL ROLE OF NF-KAPPA-B ACTIVATION IN SILICA-INDUCED INFLAMMATORY MEDIATOR PRODUCTION IN MACROPHAGES
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DOI：
10.1006/bbrc.1995.2383
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发表时间：
1995-09-25
影响因子：
3.1
通讯作者：
DEMERS, LM
中科院分区：
文献类型：
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作者：
CHEN, F;SUN, SC;DEMERS, LM
在这份报告中，我们证明了NF-κ B B，一种普遍存在的转录因子，在小鼠巨噬细胞系RAW 264.7中二氧化硅诱导的炎症介质产生中起着至关重要的作用。与脂多糖（LPS）的作用相比，二氧化硅在刺激后早期介导了更强的NF-κ B B p50/p50同源二聚体的激活。此外，二氧化硅和LPS对NF-κ B的激活似乎是由不同的信号转导途径介导的。二氧化硅和LPS均增加这些细胞中环氧合酶II、诱导型一氧化氮合酶、肿瘤坏死因子-α和白细胞介素-1 α的mRNA表达。这种表达随着NF-κ B活化的抑制而沿着减弱。(C)出版社：Academic Press
In this report, we demonstrate that NF-kappa B, a ubiquitous transcription factor, plays an essential role in silica-induced inflammatory mediator production in the mouse macrophage cell line RAW 264.7. Compared to the effect of lipopolysaccharide (LPS), silica mediated a stronger activation of NF-kappa B p50/p50 homodimer at early phase of poststimulation. Furthermore, activation of NF-kappa B by silica and LPS appears to be mediated by different signal transduction pathways. Both silica and LPS increased mRNA expression in these cells for cyclooxygenase II, inducible nitric oxide synthase, tumor necrosis factor-alpha and interleukin-1 alpha. This expression was attenuated along with the inhibition of NF-kappa B activation. (C) 1995 Academic Press, Inc.