Relaxin production by the porcine ovarian graafian follicle in vitro.
Relaxin production by the porcine ovarian graafian follicle in vitro.
复制标题
猪卵巢格拉夫卵泡在体外产生松弛素。
作者：
G. Bryant;R. Jeffrey;M. Ralph;R. Seamark
免疫反应性rela.xin显示存在于从周期性和妊娠母猪的个体卵巢收集的猪卵泡液池中。在周期性动物中，最高松弛素水平从含有活性黄体的卵巢获得，但在周期的任何阶段，卵泡液的松弛素含量接近妊娠母猪卵巢中的松弛素含量。在Sephadex C-SO上分离卵泡液，显示至少三个不同分子量的免疫反应性松弛素峰：> 40，000，20，000，和一个对应于真正的松弛素（6300）。当以类似方式分级时，怀孕母猪的血清含有更多的最高分子量免疫反应性松弛素，而只有20%的免疫反应性与6300松弛素相关。分离的卵泡和卵泡组织在培养过程中长时间释放松弛素。妊娠母猪组织的松弛素体外分泌量显著高于非妊娠母猪组织。类固醇激素和松弛素释放之间的关系的检查显示，卵泡壁段，冲洗卵泡液，释放更多的松弛素和类固醇比完整的卵泡。在检查的类固醇中，松弛素的模式最类似于孕酮的模式，孕酮在培养48小时后随着组织的自发黄体化而增加。通过添加类固醇生物合成的抑制剂，在这些培养物中进一步研究类固醇激素和松弛素生物合成之间的关系。氨鲁米特有效地减少生产的所有类固醇研究没有显着减少松弛素的总产量在培养期间。雄甾烷和雄三烯3，17-二酮在减少类固醇生成方面仅部分有效，但引起总松弛素产生的显著减少。因此，卵泡组织在体外合成并分泌松弛素，但其细胞来源、与类固醇生成的关系以及释放途径仍有待确定。
Immunoreactive rela.xin was shown to be present in pools of porcine follicular fluid collected from individual ovaries from cyclic and pregnant sows. In the cyclic animals, the highest relaxin level was obtained from ovaries containing active corpora lutes, but at no stage during the cycle did relaxin content of follicular fluid approach that found in ovaries of pregnant sows. Fractionation of follicular fluid on Sephadex C-SO revealed at least three immunoreactive relaxin peaks of differing molecular weight: >40,000, 20,000, and one corresponding to authentic relaxin (6300). Serum of pregnant sows when fractionated in a similar fashion contained more of the highest molecular weight immunoreactive relaxin while only ‘\‘20% of the immunoreactivity was associated with 6300 relaxin. Isolated ovarian follicles and follicular tissue released relaxin throughout prolonged periods in culture. Relaxin output in vitro was significantly higher for tissue obtained from pregnant than from nonpregnant sows. Examination of the relationship between steroid hormone and relaxin release revealed that follicle wall segments, washed free of follicular fluid, released both more relaxin and steroids than did intact follicles. Of the steroids examined, the pattern of relaxin most resembled that found for progesterone, which showed an increase after 48 h of culture as the tissue underwent spontaneous luteinization. The relationship between steroid hormone and relaxin biosynthesis was further studied in these cultures by the addition of inhibitors of steroid biosynthesis. Aminoglutethimide effectively reduced production of all steroids studied without significantly reducing the total production of relaxin over the culture period. Tniostane and androstriene 3, 17-dione were only partially effective in reducing steroidogenesis but caused a significant reduction in total relaxin production. Follicular tissue therefore synthesizes and secretes relaxin in vitro, but the cellular origin, the relationship with steroidogenesis, and the routes of release remain to be established.