Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells
Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells
复制标题
DOI：
10.1186/1756-3305-5-229
复制
发表时间：
2012-10-10
影响因子：
3.2
通讯作者：
Pedra, Joao H. F.
中科院分区：
文献类型：
--
作者：
Chen, Gang;Severo, Maiara S.;Pedra, Joao H. F.
背景资料：肩突硬蜱唾液具有免疫调节、麻醉和抗凝血作用，可将病原体传播给哺乳动物宿主。然而，我如何。方法：用病原体相关分子模式（pathogenic associated molecular patterns，PAMPs）和嗜吞噬细胞无形体（Anaplasma phagocytophilum）刺激骨髓源性巨噬细胞（BMDMs）。嗜吞噬细胞菌在存在和不存在I的情况下测量细胞因子分泌。肩胛肌唾液在存在和不存在I.结果：I.肩胛肌唾液和白细胞介素（IL-8）含量的变化。肩胛肌唾液抑制巨噬细胞在Toll样（TLR）和Nod样受体（NLR）信号传导途径刺激期间的炎性细胞因子分泌。I.肩胛唾液对免疫细胞的作用不限于鼠巨噬细胞，因为在TNF-α刺激人外周血单核细胞后观察到白细胞介素（IL）-8水平降低。I.肩胛肌唾液还减轻了在用A.结论：这些结果表明，I。肩胛肌可以抑制立克次体在载体-宿主界面传播期间的炎性细胞因子分泌。
Background: Ixodes scapularis saliva enables the transmission of infectious agents to the mammalian host due to its immunomodulatory, anesthetic and anti-coagulant properties. However, how I. scapularis saliva influences host cytokine secretion in the presence of the obligate intracellular rickettsial pathogen Anaplasma phagocytophilum remains elusive.Methods: Bone marrow derived macrophages (BMDMs) were stimulated with pathogen associated molecular patterns (PAMPs) and A. phagocytophilum. Cytokine secretion was measured in the presence and absence of I. scapularis saliva. Human peripheral blood mononuclear cells (PBMCs) were also stimulated with Tumor Necrosis Factor (TNF)-alpha in the presence and absence of I. scapularis saliva and interleukin (IL)-8 was measured.Results: I. scapularis saliva inhibits inflammatory cytokine secretion by macrophages during stimulation of Toll- like (TLR) and Nod-like receptor (NLR) signaling pathways. The effect of I. scapularis saliva on immune cells is not restricted to murine macrophages because decreasing levels of interleukin (IL)-8 were observed after TNF-alpha stimulation of human peripheral blood mononuclear cells. I. scapularis saliva also mitigates pro-inflammatory cytokine response by murine macrophages during challenge with A. phagocytophilum.Conclusions: These findings suggest that I. scapularis may inhibit inflammatory cytokine secretion during rickettsial transmission at the vector-host interface.