Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction
Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction
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DOI：
10.1634/stemcells.2007-0484
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发表时间：
2008-01-01
期刊：
影响因子：
5.2
通讯作者：
Sepulveda, Pilar
中科院分区：
文献类型：
--
作者：
Gandia, Carolina;Arminan, Ana;Sepulveda, Pilar
牙髓干细胞（dental pulp stem cells，DPSC）是一种具有自我更新和多向分化能力的前体细胞，可分泌多种促血管生成因子和抗凋亡因子。为了检查这些细胞是否在心肌梗死（MI）的修复中具有治疗潜力，用编码绿色荧光蛋白（GFP）的逆转录病毒感染DPSC并离体扩增。通过冠状动脉结扎诱导心肌梗死后7天，在裸大鼠心肌内注射1.5 × 10（6）GFP-DPSC。在4周时，细胞处理的动物显示出心脏功能的改善，通过前壁增厚左心室分数面积变化的百分比变化观察到，与梗死面积的减少平行。在梗死灶内未观察到GFP（+）内皮细胞、平滑肌细胞或心肌细胞的组织学证据。然而，相对于对照处理的动物，血管生成增加。总之，这些数据表明，DPSC可以提供一种新的替代细胞群的心脏修复，至少在急性心肌梗死的设置。
Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 x 10(6) GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP(+) endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI.