Characterization of sulfide oxidation and optimization of sulfate production by a thermophilic Paenibacillus naphthalenouorans LYH-3 isolated from sewage sludge composting
Characterization of sulfide oxidation and optimization of sulfate production by a thermophilic Paenibacillus naphthalenouorans LYH-3 isolated from sewage sludge composting
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从污水污泥堆肥中分离出的嗜热萘类芽孢杆菌 LYH-3 的硫化物氧化特性和硫酸盐生产优化
DOI：
10.1016/j.jes.2021.12.030
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发表时间：
2022-04-07
影响因子：
6.9
通讯作者：
Zhou, Yujie
中科院分区：
文献类型：
--
作者：
Chen, Li;Li, Weiguang;Zhou, Yujie
污泥堆肥过程中产生的含硫恶臭可以通过硫化物氧化菌来控制，但中温菌在高温堆肥过程中表现出失活。为了研究并鉴定能将硫化物氧化成硫酸盐的耐热细菌，从城市污泥堆肥中分离到一株异养菌，经鉴定为嗜萘类芽孢杆菌（Paenibacillus naphthalenovorans）LYH-3。通过考察各种环境因素对硫化物氧化能力的影响，优化了硫酸盐的生产工艺，采用BoxBehnken试验设计，在pH值为7.34、转速为161.14 r/min、接种量为5.83%的条件下，硫酸盐的产率最高，为27.35% ± 0.86%。硫化物系列底物试验结果表明，菌株LYH-3在硫化物浓度为400 mg/L时仍能存活，在硫化物浓度为50 mg/L时，其硫化物去除率最高，为88.79% ± 0.35%。生长动力学分析显示在50 ℃培养22小时后的最大比生长速率μ m（0.5274 hr-1）。硫化物醌氧化还原酶（0.369 +/- 0.052 U/mg）和硫双加氧酶（0.255 +/- 0.014 U/mg）的最高酶活性均在40 ℃下获得，并且亚硫酸盐受体氧化还原酶（1.302 +/- 0.035 U/mg）的最高酶活性在50 ℃下评估。结果表明，嗜萘假单胞菌在高温条件下具有快速生长和高效氧化硫化物的能力，在污泥堆肥高温阶段的含硫恶臭控制中具有潜在的应用前景。(c)2022中国科学院生态环境科学研究中心由爱思唯尔公司出版
The sulfur-containing odor emitted from sludge composting could be controlled by sulfide oxidizing bacteria, yet mesophilic strains show inactivation during the thermophilic stage of composting. Aimed to investigate and characterize the thermotolerant bacterium that could oxidize sulfide into sulfate, a heterotrophic strain was isolated from sewage sludge composting and identified as Paenibacillus naphthalenovorans LYH-3. The effects of various environmental factors on sulfide oxidation capacities were studied to optimize the sulfate production, and the highest production rate (27.35% +/- 0.86%) was obtained at pH 7.34, the rotation speed of 161.14 r/min, and the inoculation amount of 5.83% by employing BoxBehnken design. The results of serial sulfide substrates experiments indicated that strain LYH-3 could survive up to 400 mg/L of sulfide with the highest sulfide removal rate (88.79% +/- 0.35%) obtained at 50 mg/L of sulfide. Growth kinetic analysis presented the maximum specific growth rate mu m (0.5274 hr-1 ) after 22 hr cultivation at 50 degrees C. The highest enzyme activities of sulfide quinone oxidoreductase (0.369 +/- 0.052 U/mg) and sulfur dioxygenase (0.255 +/- 0.014 U/mg) were both obtained at 40 degrees C, and the highest enzyme activity of sulfite acceptor oxidoreductase (1.302 +/- 0.035 U/mg) was assessed at 50 degrees C. The results indicated that P. naphthalenovorans possessed a rapid growth rate and efficient sulfide oxidation capacities under thermophilic conditions, promising a potential application in controlling sulfur-containing odors during the thermophilic stage of sludge composting. (c) 2022 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.