Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity
Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity
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DOI：
10.1080/17435390701381596
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发表时间：
2007-01-01
期刊：
影响因子：
5
通讯作者：
Castranova, Vincent
中科院分区：
文献类型：
--
作者：
Sager, Tina M.;Porter, Dale W.;Castranova, Vincent
纳米颗粒在溶液中聚集和结块，使得难以准确地递送它们用于体内或体外实验。因此，进行实验以确定悬浮纳米尺寸颗粒的最佳方法。将超细和精细炭黑和二氧化钛悬浮于磷酸盐缓冲盐水（PBS）、大鼠和小鼠支气管肺泡灌洗液（BALF）以及含有二棕榈酰磷脂酰胆碱（DPPC）和/或小鼠血清白蛋白的PBS中。为了评估和比较这些各种悬浮介质如何分散纳米颗粒，使用光学显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）拍摄图像。该研究的结果表明，PBS不是制备纳米颗粒悬浮液的令人满意的介质。然而，BALF是悬浮纳米颗粒的优良介质。在BALF中发现的浓度下，单独使用含蛋白质的PBS或DPPC不能产生令人满意的颗粒分散。然而，含PBS的蛋白质加DPPC是令人满意的，尽管不如BALF有效。
Nanoparticles agglomerate and clump in solution, making it difficult to accurately deliver them for in vivo or in vitro experiments. Thus, experiments were conducted to determine the best method to suspend nanosized particles. Ultrafine and fine carbon black and titanium dioxide were suspended in phosphate buffered saline (PBS), rat and mouse bronchoalveolar lavage fluid (BALF), and PBS containing dipalmitoyl phosphatidylcholine (DPPC) and/or mouse serum albumin. To assess and compare how these various suspension media dispersed the nanoparticles, images were taken using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results of this study show that PBS is not a satisfactory medium to prepare nanoparticle suspensions. However, BALF was an excellent media in which to suspend nanoparticles. The use of PBS containing protein or DPPC alone, in concentrations found in BALF, did not result in satisfactory particle dispersion. However, PBS-containing protein plus DPPC was satisfactory, although less effective than BALF.