Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions
Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions
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DOI：
10.1073/pnas.95.13.7705
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Nagao, S
中科院分区：
文献类型：
--
作者：
Katoh, A;Kitazawa, H;Nagao, S
使用红外电视摄像系统测量了两种长期制备的小鼠品系的反射性眼球运动的动态特征。水平前庭眼反射（HVOR）和水平视动反应（HOKR）分别由转盘的正弦振荡引起，在黑暗中，在0.11-0.50赫兹下10度（峰到峰），在光下，通过格子屏幕在0.11-0.17赫兹下5-20度的正弦振荡。 C57BL/6 小鼠的 HVOR 和 HOKR 的增益和相位几乎与兔子和大鼠相同，而 129/Sv 小鼠的 HVOR 增益非常低，HOKR 的相位滞后中等，表明存在固有的感觉运动异常。通过持续屏幕振荡在 C57BL/6 小鼠中检查 HOKR 的适应性。当屏幕以 0.17 Hz 振动 10 度，引起足够的视网膜滑动时，HOKR 的增益在 1 小时内平均增加 0.08，而引起相对较小或不引起视网膜滑动的刺激对增益的影响很小。局部应用0.1%鹅膏蕈酸引起的絮状损伤和腹腔注射引起的下橄榄核损伤。在 C57BL/6 小鼠中注射 3-乙酰吡啶对 HVOR 和 HOKR 的动态特性几乎没有影响，但消除了 HOKR 的适应性。这些结果表明，橄榄絮状系统在小鼠眼反射的适应性控制中起着重要作用，正如其他动物物种所表明的那样。所提供的数据为分析基因工程小鼠的反射性眼球运动提供了基础。
The dynamic characteristics of reflex eye movements were measured in two strains of chronically prepared mice by using an infrared television camera system. The horizontal vestibule-ocular reflex (HVOR) and horizontal optokinetic response (HOKR) were induced by sinusoidal oscillations of a turntable, in darkness, by 10 degrees (peak to peak) at 0.11-0.50 Hz and of a checked-pattern screen, in light, by 5-20 degrees at 0.11-0.17 Hz, respectively. The gains and phases of the HVOR and HOKR of the C57BL/6 mice were nearly equivalent to those of rabbits and rats, whereas the 129/Sv mice exhibited very low gains in the HVOR and moderate phase lags in the HOKR, suggesting an inherent sensory-motor anomaly. Adaptability of the HOKR was examined in C57BL/6 mice by sustained screen oscillation. When the screen was oscillated by 10 degrees at 0.17 Hz, which induced sufficient retinal slips, the gain of the HOKR increased by 0.08 in 1 h on average, whereas the stimuli that induced relatively small or no retinal slips affected the gain very little. Lesions of the flocculi induced by local applications of 0.1% ibotenic acid and lesions of the inferior olivary nuclei induced by i.p. injection of 3-acetylpyridine in C57BL/6 mice little affected the dynamic characteristics of the HVOR and HOKR, but abolished the adaptation of the HOKR, These results indicate that the olivo-floccular system plays an essential role in the adaptive control of the ocular reflex in mice, as suggested in other animal species. The data presented provide the basis for analyzing the reflex eye movements of genetically engineered mice.