Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes
Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes
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DOI：
10.2116/analsci.20n032
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发表时间：
2021-07-01
影响因子：
1.6
通讯作者：
Kamei, Ken-ichiro
中科院分区：
文献类型：
--
作者：
Abdalkader, Rodi;Chaleckis, Romanas;Kamei, Ken-ichiro
由于细胞培养基（CCM）体积小，微生理模型中的代谢组分析是一项挑战。在这里，我们报告了一种基于LC-MS的非靶向代谢组学方案，用于检测CCM微量样品中的肝细胞外代谢产物。使用单一LC-MS方法，我们检测到57种代谢物，其中27种在72小时孵育后显示>2倍的变化。我们证明，微尺度CCM样品可用于模拟代谢物强度的微生理时间动态。
Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.