Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines
Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines
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DOI：
10.1007/s00122-015-2551-8
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发表时间：
2015-09-01
影响因子：
5.4
通讯作者：
Boddupalli, Prasanna M.
中科院分区：
文献类型：
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作者：
Nair, Sudha K.;Babu, Raman;Boddupalli, Prasanna M.
在1号染色体87 Mb处，将MSV抗性主要QTL Msv1定位在0.87 cM区间，开发出预测精度较高的生产标记。玉米条纹病毒（MSV）病是撒哈拉以南非洲（SSA）地区的一种破坏性病害，造成玉米产量的重大损失。对MSV的抗性以前被定位到1号染色体上的一个与种质和环境无关的主要QTL （Msv1）和基因组其他地方的几个次要位点。本研究利用CML206 × CML312的F(2)大群体，在1号染色体0.87 cM区间，通过QTL等基因重组策略对Msv1进行了精细定位。利用高密度测序基因分型（GBS）标记，在CIMMYT 278个热带/亚热带育种品系的DTMA（非洲耐旱玉米）关联图谱中进行了全基因组关联研究。本研究在1号染色体（B73 RefGen_V2） 82 ~ 93 Mb区域发现19个snp， P < 1.00E-04，与Msv1精细定位区域重合。单倍型趋势回归发现了一个与MSV应答显著相关的单倍型区。1号染色体87 Mb单倍型块中的3个snp预测已知MSV感染反应的育种品系的疾病反应的准确度为0.94。在两个双亲本群体中，抗性Msv1单倍型的选择表明，与易感单倍型相比，抗性Msv1单倍型在1-5的评分范围内减少了1.03-1.39个单位。高通量KASP检测方法已经开发出来，可以在育种管道中对这三个snp进行常规标记筛选，以检测MSV抗性。
Msv1 , the major QTL for MSV resistance was delimited to an interval of 0.87 cM on chromosome 1 at 87 Mb and production markers with high prediction accuracy were developed.Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F (2) population of CML206 x CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breeding lines from CIMMYT using the high-density genotyping-by-sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03-1.39 units on a rating scale of 1-5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistance.