NUCLEOTIDE-SEQUENCE OF THE GENE FOR HUMAN-PROTHROMBIN
NUCLEOTIDE-SEQUENCE OF THE GENE FOR HUMAN-PROTHROMBIN
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DOI：
10.1021/bi00393a033
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发表时间：
1987-09-22
期刊：
影响因子：
2.9
通讯作者：
DAVIE, EW
中科院分区：
文献类型：
--
作者：
DEGEN, SJF;DAVIE, EW
从人类基因组DNA文库中筛选出编码人凝血酶原的基因和编码人凝血酶原的基因。81个阳性。兰布达。对噬菌体进行了鉴定，并选择了三个进行进一步鉴定。这三个噬菌体与凝血酶原基因的5‘’和/或3‘’探针杂交。测定了人凝血酶原基因21千碱基的完整DNA序列，其中包括先前测序的4.9千碱基区。人类凝血酶原基因包含14个外显子，由13个插入序列隔开。外显子的大小从25到315个碱基对，而内含子的大小从84到9447个碱基对。该基因90%由插入序列组成。除了在插入序列L的5‘端存在GC而不是GT外，所有内含子剪接连接都与其他真核基因中发现的序列一致，在几个插入序列中的簇内发现了30个Alu重复DNA拷贝和两个部分KpnI重复序列，这些重复序列占该基因DNA序列的40%。然后将基因内含子的大小、分布和序列同源性与其他维生素K依赖蛋白和其他几种丝氨酸蛋白酶的基因进行比较。
A human genomic DNA libary was screened for the gene coding for human prothrombin with a cDNA coding for the human protein. Eighty-one positive .lambda. phage were identified, and three were chosen for further characterization. These three phage hybridized with 5'' and/or 3'' probes prepared from the prothrombin cDNA. The complete DNA sequence of 21 kilobases of the human prothrombin gene was determined and included a 4.9-kilobase region that was previously sequenced. The gene for human prothrombin contains 14 exons separated by 13 intervening sequences. The exons range in size from 25 to 315 base pairs, while the introns range from 84 to 9447 base pairs. Ninety percent of the gene is composed of intervening sequence. All the intron splice junctions are consistent with sequences found in other eukaryotic genes, except for the presence of GC rather than GT on the 5'' end of intervening sequence L. Thirty copies of Alu repetitive DNA and two copies of partial KpnI repeats were identified in clusters within several of the intervening sequences, and these repeats represent 40% of the DNA sequence of the gene. The size, distribution, and sequence homology of the introns within the gene were then compared to those of the genes for the other vitamin K dependent proteins and several other serine proteases.