Correlation of perinatal transmission of human immunodeficiency virus type 1 with maternal viremia and lymphocyte phenotypes.
Correlation of perinatal transmission of human immunodeficiency virus type 1 with maternal viremia and lymphocyte phenotypes.
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1 型人类免疫缺陷病毒围产期传播与母体病毒血症和淋巴细胞表型的相关性。
DOI：
10.1016/s0022-3476(05)83274-3
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Tao,PT
中科院分区：
文献类型：
--
作者：
Borkowsky,W;Krasinski,K;Cao,Y;Ho,D;Pollack,H;Moore,T;Chen,SH;Allen,M;Tao,PT
目的探讨人类免疫缺陷病毒（human immunodeficiency virus，HIV）母婴传播是否与HIV感染量增加、CD 4 +T细胞比例降低或CD 8 +T细胞比例升高有关。血浆用于通过培养进行定量病毒血症的测量，随后测量定量HIV-1核糖核酸水平。分析这些测量结果与婴儿艾滋病毒传播的关系，这发生在四分之一的病例中。儿童也进行了研究，以确定是否检测到HIV-1的出生或不，直到1至8周的生命。ResultsHIV-1感染的外周血单核细胞的克隆频率增加被发现在母亲的感染儿童;五倍少的细胞需要一个积极的文化结果（中位数细胞数为104.5vs 105.2;P=0.008）。在出生时有感染证据的婴儿的母亲中，感染细胞的频率高于出生时无感染证据的感染婴儿的母亲（p<0.05）。在10%的培养物中测量了血浆病毒血症，而不考虑母亲是否将病毒传播给婴儿。通过支链DNA方法检测到的核糖核酸水平增加在感染儿童的母亲中比在未感染儿童的母亲中更常见（45%对17%）。在这两组women.ConclusionsIncreased病毒血症是存在于母亲谁传播艾滋病毒给他们的后代的CD 4+和CD 8 +T细胞的比例是不可区分的。这一变量可用于选择将艾滋病毒传播给后代的风险最高的妇女进行治疗，以将艾滋病毒负担减少五倍。
ObjectiveTo determine whether maternal transmission of human immunodeficiency virus (HIV) is correlated with increased quantities of HIV, decreased frequencies of CD4+T cells, or increased levels of CD8+T cells in the transmitting mother.MethodsPeripheral blood obtained from HIV-infected women at different times during pregnancy was used to measure quantitative cell-associated HIV-1 and CD3+CD4+and CD3+CD8+proportions; the plasma was used to perform measurements of quantitative viremia by culture and subsequently to measure quantitative HIV-1 ribonucleic acid levels. These measurements were analyzed with respect to their association with HIV transmission to the baby, which occurred in one fourth of the cases. The children were also studied to determine whether HIV-1 was detected near birth or not until 1 to 8 weeks of life.ResultsIncreased clonal frequencies of HIV-1-infected peripheral blood mononuclear cells were found in mothers of infected children; fivefold fewer cells were required for a positive culture result (median cell numbers of 104.5vs 105.2;p=0.008). Higher frequencies of infected cells were seen in mothers of babies with evidence of infection at birth than in mothers of infected babies without evidence of infection at birth (p<0.05). Plasma viremia was measured in 10% of cultures without regard to whether the mothers transmitted virus to their babies. Increased levels of ribonucleic acid as detected by the branched-chain DNA method were measurable more often (45% vs 17%) in the mothers of infected children than in mothers of uninfected children. Proportions of CD4+and CD8+T cells were indistinguishable in these two groups of women.ConclusionsIncreased viremia was present in mothers who transmitted HIV to their offspring. This variable could be used to select women at highest risk of transmitting HIV to their offspring for treatment to decrease the HIV burden fivefold.