RELATIVE EXON AFFINITIES AND SUBOPTIMAL SPLICE-SITE SIGNALS LEAD TO NON-EQUIVALENCE OF 2 CASSETTE EXON
RELATIVE EXON AFFINITIES AND SUBOPTIMAL SPLICE-SITE SIGNALS LEAD TO NON-EQUIVALENCE OF 2 CASSETTE EXON
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DOI：
10.1093/nar/23.17.3585
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发表时间：
1995-09-11
影响因子：
14.9
通讯作者：
KOSIK, KS
中科院分区：
文献类型：
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作者：
ANDREADIS, A;BRODERICK, JA;KOSIK, KS
Tau是一种微管相关蛋白，其转录物在哺乳动物神经系统中经历复杂的调控剪接。该基因的外显子2和3是选择性剪接盒，其中外显子3从不独立于外显子2出现。tau小基因构建体在细胞中的表达表明外显子2类似于组成型外显子，而连接到外显子3的次优分支点抑制外显子3包含在mRNA中。两个tau外显子的剪接由它们彼此的相对亲和力相对于它们的侧翼外显子对它们的亲和力控制。
Tau is a microtubule-associated protein whose transcript undegoes complex regulated splicing in the mammalian nervous system. Exons 2 and 3 of the gene are alternatively spliced cassettes in which exon 3 never appears independently of exon 2. Expression of tau minigene constructs in cells indicate that exon 2 resembles a constitutive exon, while a suboptimal branch point connected to exon 3 inhibits inclusion of exon 3 in the mRNA, Splicing of the two tau exons is controlled by their relative affinities for each other versus the affinities of their flanking exons for them.